Proliferative Effect of Tilapia Fish (Oreochromis niloticus) Lectin on BALB/c Mice Splenocytes.

Autor:	Cardoso da Silva CD; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Lagos de Melo CM; Departamento de Antibióticos, Centro de Biociências, Recife, Pernambuco, Brazil., Matoso Maciel Carvalho EV; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Lino da Silva MA; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Bezerra RF; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Guerra Dos Santos AJ; Departamento de Engenharia de Pesca, Universidade Federal Rural de Pernambuco/UFRPE, Pernambuco, Brazil., Alves Pereira VR; Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ, Pernambuco, Brazil., Breitenbach Barroso Coelho LC; Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Jazyk:	angličtina
Zdroj:	Protein and peptide letters [Protein Pept Lett] 2019; Vol. 26 (12), pp. 887-892.
DOI:	10.2174/0929866526666190911144057
Abstrakt:	Background: Lectins have been studied in recent years due to their immunomodulatory activities. Objective: We purified a lectin named OniL from tilapia fish (Oreochromis niloticus) and here we analyzed the cell proliferation and cytokine production in Balb/c mice splenocytes. Methods: Cells were stimulated in vitro in 24, 48, 72 hours and 6 days with different concentrations of OniL and Con A. Evaluation of cell proliferation was performed through [3H]-thymidine incorporation, cytokines were investigated using ELISA assay and cell viability assay was performed by investigation of damage through signals of apoptosis and necrosis. Results: OniL did not promote significant cell death, induced high mitogenic activity in relation to control and Con A and stimulated the cells to release high IL-2 and IL-6 cytokines. Conclusion: These findings suggest that, like Con A, OniL lectin can be used as a mitogenic agent in immunostimulatory assays. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
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