Spatiotemporal Gradient of Cortical Neuron Death Contributes to Microcephaly in Knock-In Mouse Model of Ligase 4 Syndrome.

Autor:	Lun MP; Department of Pathology, Boston Children's Hospital, Boston, Massachusetts; Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts., Shannon ML; Department of Pathology, Boston Children's Hospital, Boston, Massachusetts., Keles S; Department of Pediatric Allergy and Immunology, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey., Reisli I; Department of Pediatric Allergy and Immunology, Meram Medical Faculty, Necmettin Erbakan University, Konya, Turkey., Luche N; Division of Immunology, Boston Children's Hospital, Boston, Massachusetts., Ryan D; Division of Immunology, Boston Children's Hospital, Boston, Massachusetts., Capuder K; Division of Immunology, Boston Children's Hospital, Boston, Massachusetts., Notarangelo LD; Immune Deficiency Genetics Section, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland., Lehtinen MK; Department of Pathology, Boston Children's Hospital, Boston, Massachusetts. Electronic address: maria.lehtinen@childrens.harvard.edu.
Jazyk:	angličtina
Zdroj:	The American journal of pathology [Am J Pathol] 2019 Dec; Vol. 189 (12), pp. 2440-2449. Date of Electronic Publication: 2019 Sep 18.
DOI:	10.1016/j.ajpath.2019.08.010
Abstrakt:	Cells of the developing central nervous system are particularly susceptible to formation of double-stranded DNA breaks (DSBs) arising from physiological and/or environmental insults. Therefore, efficient repair of DSBs is especially vital for maintaining cellular health and proper functioning in the developing brain. Here, increased expression of DSB initiating and nonhomologous end joining repair machinery in newborn neurons in the developing brains of both mouse and human are demonstrated. In parallel, the first characterization is provided of the brain phenotype in the Lig4 R278H/R278H (Lig4 R/R ) mouse model of DNA Ligase 4 (LIG4) syndrome, in which a hypomorphic Lig4 mutation, originally identified in patients, impedes nonhomologous end joining. It is shown that Lig4 R/R mice develop nonprogressive microcephaly, resulting primarily from apoptotic death of newborn neurons that is both spatially and temporally specific during peak cortical neurogenesis. This apoptosis leads to a reduction in neurons throughout the postnatal cerebral cortex, but with a more prominent impact on those of the lower cortical layers. Together, these findings begin to uncover the pathogenesis of microcephaly in LIG4 syndrome and open avenues to more focused investigations on the critical roles of DSB formation and repair in vulnerable neuronal populations of the brain. (Copyright © 2019 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu