Formation of Pseudomonas aeruginosa Biofilms in Full-thickness Scald Burn Wounds in Rats.

Autor: Brandenburg KS; Dental and Craniofacial Trauma Research and Tissue Regeneration Department, United States Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam, Houston, Texas, 78234, USA., Weaver AJ Jr; Dental and Craniofacial Trauma Research and Tissue Regeneration Department, United States Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam, Houston, Texas, 78234, USA., Karna SLR; Dental and Craniofacial Trauma Research and Tissue Regeneration Department, United States Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam, Houston, Texas, 78234, USA., You T; Dental and Craniofacial Trauma Research and Tissue Regeneration Department, United States Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam, Houston, Texas, 78234, USA., Chen P; Dental and Craniofacial Trauma Research and Tissue Regeneration Department, United States Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam, Houston, Texas, 78234, USA., Stryk SV; Dental and Craniofacial Trauma Research and Tissue Regeneration Department, United States Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam, Houston, Texas, 78234, USA., Qian L; Dental and Craniofacial Trauma Research and Tissue Regeneration Department, United States Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam, Houston, Texas, 78234, USA., Pineda U; Dental and Craniofacial Trauma Research and Tissue Regeneration Department, United States Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam, Houston, Texas, 78234, USA., Abercrombie JJ; Dental and Craniofacial Trauma Research and Tissue Regeneration Department, United States Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam, Houston, Texas, 78234, USA., Leung KP; Dental and Craniofacial Trauma Research and Tissue Regeneration Department, United States Army Institute of Surgical Research, 3650 Chambers Pass, Bldg 3610, JBSA Fort Sam, Houston, Texas, 78234, USA. kai.p.leung.civ@mail.mil.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Sep 20; Vol. 9 (1), pp. 13627. Date of Electronic Publication: 2019 Sep 20.
DOI: 10.1038/s41598-019-50003-8
Abstrakt: Using Sprague-Dawley rats (350-450 g; n = 61) and the recently updated Walker-Mason rat scald burn model, we demonstrated that Pseudomonas aeruginosa readily formed biofilms within full-thickness burn wounds. Following the burn, wounds were surface-inoculated with P. aeruginosa in phosphate-buffered saline (PBS), while sterile PBS was used for controls. On post-burn days 1, 3, 7, and 11, animals were euthanized and samples collected for quantitative bacteriology, bacterial gene expression, complete blood cell counts, histology, and myeloperoxidase activity. Robust biofilm infections developed in the full-thickness burn wounds inoculated with 1 × 10 4 CFU of P. aeruginosa. Both histology and scanning electron microscopy showed the pathogen throughout the histologic cross-sections of burned skin. Quantigene analysis revealed significant upregulation of alginate and pellicle biofilm matrix genes of P. aeruginosa within the burn eschar. Additionally, expression of P. aeruginosa proteases and siderophores increased significantly in the burn wound environment. Interestingly, the host's neutrophil response to the pathogen was not elevated in either the eschar or circulating blood when compared to the control burn. This new full-thickness burn biofilm infection model will be used to test new anti-biofilm therapies that may be deployed with soldiers in combat for immediate use at the site of burn injury on the battlefield.
Databáze: MEDLINE
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