Monitoring the Formation of Amyloid Oligomers Using Photoluminescence Anisotropy.

Autor:	Jiang B, Aliyan A; Pasargad Institute for Advanced Innovative Solutions, & Khatam University , Tehran , 1991633361 , Iran., Cook NP; Department of Chemistry , Williams College , Williamstown , Massachusetts 01267 , United States., Augustine A, Bhak G; Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Departamento de Química Orgánica , Universidade de Santiago de Compostela , 15782 Santiago de Compostela , Spain., Maldonado R, Smith McWilliams AD, Flores EM; Departamento de Química de los Materiales, Facultad de Química y Biología , Universidad de Santiago de Chile , Av. Libertador B. O'Higgins , 3363 Santiago , Chile., Mendez N; Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology , The University of Texas Health and Science Center at Houston , Houston , Texas 77030 , United States., Shahnawaz M; Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology , The University of Texas Health and Science Center at Houston , Houston , Texas 77030 , United States., Godoy FJ; Departamento de Química de los Materiales, Facultad de Química y Biología , Universidad de Santiago de Chile , Av. Libertador B. O'Higgins , 3363 Santiago , Chile., Montenegro J; Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Departamento de Química Orgánica , Universidade de Santiago de Compostela , 15782 Santiago de Compostela , Spain., Moreno-Gonzalez I; Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology , The University of Texas Health and Science Center at Houston , Houston , Texas 77030 , United States.; Dpto. Biologia Celular, Genetica y Fisiologia, Instituto de Investigacion Biomedica de Malaga-IBIMA, Facultad de Ciencias , Universidad de Malaga, Spain and Centro de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas (CIBERNED) , Madrid , Spain., Martí AA
Jazyk:	angličtina
Zdroj:	Journal of the American Chemical Society [J Am Chem Soc] 2019 Oct 02; Vol. 141 (39), pp. 15605-15610. Date of Electronic Publication: 2019 Sep 19.
DOI:	10.1021/jacs.9b06966
Abstrakt:	The formation of oligomeric soluble aggregates is related to the toxicity of amyloid peptides and proteins. In this manuscript, we report the use of a ruthenium polypyridyl complex ([Ru(bpy) 2 (dpqp)] 2+ ) to track the formation of amyloid oligomers at different times using photoluminescence anisotropy. This technique is sensitive to the rotational correlation time of the molecule under study, which is consequently related to the size of the molecule. [Ru(bpy) 2 (dpqp)] 2+ presents anisotropy values of zero when free in solution (due to its rapid rotation and long lifetime) but larger values as the size and concentration of amyloid-β (Aβ) oligomers increase. Our assays show that Aβ forms oligomers immediately after the assay is started, reaching a steady state at ∼48 h. SDS-PAGE, DLS, and TEM were used to confirm and characterize the formation of oligomers. Our experiments show that the rate of formation for Aβ oligomers is temperature dependent, with faster rates as the temperature of the assay is increased. The probe was also effective in monitoring the formation of α-synuclein oligomers at different times.
Databáze:	MEDLINE
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