A distinct transition from cell growth to physiological homeostasis in the tendon.

Autor:	Grinstein M; Center for Regenerative Medicine, Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, United States., Dingwall HL; Department of Human Evolutionary Biology, Harvard University, Cambridge, United States., O'Connor LD; Center for Regenerative Medicine, Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, United States., Zou K; Center for Regenerative Medicine, Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, United States., Capellini TD; Department of Human Evolutionary Biology, Harvard University, Cambridge, United States.; Broad Institute of Harvard and MIT, Cambridge, United States., Galloway JL; Center for Regenerative Medicine, Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, United States.; Harvard Stem Cell Institute, Cambridge, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2019 Sep 19; Vol. 8. Date of Electronic Publication: 2019 Sep 19.
DOI:	10.7554/eLife.48689
Abstrakt:	Changes in cell proliferation define transitions from tissue growth to physiological homeostasis. In tendons, a highly organized extracellular matrix undergoes significant postnatal expansion to drive growth, but once formed, it appears to undergo little turnover. However, tendon cell activity during growth and homeostatic maintenance is less well defined. Using complementary methods of genetic H2B-GFP pulse-chase labeling and BrdU incorporation in mice, we show significant postnatal tendon cell proliferation, correlating with longitudinal Achilles tendon growth. Around day 21, there is a transition in cell turnover with a significant decline in proliferation. After this time, we find low amounts of homeostatic tendon cell proliferation from 3 to 20 months. These results demonstrate that tendons harbor significant postnatal mitotic activity, and limited, but detectable activity in adult and aged stages. It also points towards the possibility that the adult tendon harbors resident tendon progenitor populations, which would have important therapeutic implications. Competing Interests: MG, HD, LO, KZ, TC, JG No competing interests declared (© 2019, Grinstein et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu