Diversity of Dromedary Camel Coronavirus HKU23 in African Camels Revealed Multiple Recombination Events among Closely Related Betacoronaviruses of the Subgenus Embecovirus.

Autor:	So RTY; School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, Republic of China., Chu DKW; School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, Republic of China., Miguel E; MIVEGEC Maladies Infectieuses et Vecteurs: Ecologie, Génétique, Evolution et Contrôle, IRD L'Institut de Recherche pour le Développement, CNRS Centre National de Recherche Scientifique, Universitè de Montpellier, Montpellier, France., Perera RAPM; School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, Republic of China., Oladipo JO; School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, Republic of China.; Department of Surgery, Faculty of Clinical Sciences, University of Ilorin, Ilorin, Nigeria., Fassi-Fihri O; Institut Agronomique et Vétérinaire, Hassan II Université, Rabat, Morocco., Aylet G; Pan African Veterinary Center of the African Union (AU-PANVAC), Debre Zeit, Ethiopia., Ko RLW; School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, Republic of China., Zhou Z; School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, Republic of China., Cheng MS; School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, Republic of China., Kuranga SA; Department of Surgery, Faculty of Clinical Sciences, University of Ilorin, Ilorin, Nigeria., Roger FL; Animal, Santé, Territoires, Risques et Ecosystèmes, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Institut National de la Recherche Agronomique, Université de Montpellier, Montpellier, France.; Kasetsart University, Bangkok, Thailand., Chevalier V; Animal, Santé, Territoires, Risques et Ecosystèmes, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Institut National de la Recherche Agronomique, Université de Montpellier, Montpellier, France.; Institut Pasteur du Cambodge, Phnom Penh, Cambodia., Webby RJ; St. Jude Children's Research Hospital, Memphis, Tennessee, USA., Woo PCY; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, Republic of China., Poon LLM; School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, Republic of China., Peiris M; School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, Republic of China malik@hku.hk.
Jazyk:	angličtina
Zdroj:	Journal of virology [J Virol] 2019 Nov 13; Vol. 93 (23). Date of Electronic Publication: 2019 Nov 13 (Print Publication: 2019).
DOI:	10.1128/JVI.01236-19
Abstrakt:	Genetic recombination has frequently been observed in coronaviruses. Here, we sequenced multiple complete genomes of dromedary camel coronavirus HKU23 (DcCoV-HKU23) from Nigeria, Morocco, and Ethiopia and identified several genomic positions indicative of cross-species virus recombination events among other betacoronaviruses of the subgenus Embecovirus (clade A beta-CoVs). Recombinant fragments of a rabbit coronavirus (RbCoV-HKU14) were identified at the hemagglutinin esterase gene position. Homolog fragments of a rodent CoV were also observed at 8.9-kDa open reading frame 4a at the 3' end of the spike gene. The patterns of recombination differed geographically across the African region, highlighting a mosaic structure of DcCoV-HKU23 genomes circulating in dromedaries. Our results highlighted active recombination of coronaviruses circulating in dromedaries and are also relevant to the emergence and evolution of other betacoronaviruses, including Middle East respiratory syndrome coronavirus (MERS-CoV). IMPORTANCE Genetic recombination is often demonstrated in coronaviruses and can result in host range expansion or alteration in tissue tropism. Here, we showed interspecies events of recombination of an endemic dromedary camel coronavirus, HKU23, with other clade A betacoronaviruses. Our results supported the possibility that the zoonotic pathogen MERS-CoV, which also cocirculates in the same camel species, may have undergone similar recombination events facilitating its emergence or may do so in its future evolution. (Copyright © 2019 American Society for Microbiology.)
Databáze:	MEDLINE
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