Isolation and Identification of Lactic Acid Bacteria Probiotic Culture Candidates for the Treatment of Salmonella enterica Serovar Enteritidis in Neonatal Turkey Poults.

Autor: Arreguin-Nava MA; Eco-Bio LLC, Fayetteville 72701, AR, USA. marreguin@euxxis.bio., Hernández-Patlán D; Laboratorio 5: LEDEFAR, Unidad de Investigación Multidisciplinaria, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México (UNAM), Cuautitlán Izcalli Estado de México 54714, México. danielpatlan@comunidad.unam.mx., Solis-Cruz B; Laboratorio 5: LEDEFAR, Unidad de Investigación Multidisciplinaria, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México (UNAM), Cuautitlán Izcalli Estado de México 54714, México. bruno_sc@comunidad.unam.mx., Latorre JD; Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. juandlatorre@gmail.com., Hernandez-Velasco X; Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Cd. de Mexico 04510, Mexico. xochitl_h@yahoo.com., Tellez G Jr; Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. memotellez98@gmail.com., El-Ashram S; School of Life Science and Engineering, Foshan University, Foshan Guangdong 528231, China. saeed_elashram@yahoo.com.; Faculty of Science, Kafrelsheikh University, Kafr el-Sheikh, 33516, Egypt. saeed_elashram@yahoo.com., Hargis BM bhargis@uark.edu; Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA., Tellez-Isaias G; Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. gtellez@uark.edu.
Jazyk: angličtina
Zdroj: Animals : an open access journal from MDPI [Animals (Basel)] 2019 Sep 17; Vol. 9 (9). Date of Electronic Publication: 2019 Sep 17.
DOI: 10.3390/ani9090696
Abstrakt: The effect of Lactobacillus spp.-based probiotic candidates on Salmonella enterica serovar Enteritidis (SE) colonization was evaluated in two separate experiments. In each experiment, sixty-one day-of-hatch female turkey poults were obtained from a local hatchery. In both experiments, poults were challenged via oral gavage with 10 4 cfu/poult of SE and randomly allocated to one of two groups ( n = 30 poults): (1) the positive control group and (2) the probiotic treated group. Heated brooder batteries were used for housing each group separately and poults were allowed ad libitum access to water and unmedicated turkey starter feed. 1 h following the SE challenge, poults were treated with 10 6 cfu/poult of probiotic culture via oral gavage or phosphate-buffered saline （PBS）to control groups. A total of 24 h post-treatment, poults were euthanized and the ceca and cecal tonsils from twenty poults were collected aseptically for SE recovery. In both trials, a significant reduction in the incidence and log 10 cfu/g of SE were observed in poults treated with the probiotic when compared with control poults ( p ≤ 0.05). The results of the present study suggest that the administration of this lactic acid-producing bacteria (LAB)-based probiotic 1 h after an SE challenge can be useful in reducing the cecal colonization of this pathogen in neonatal poults.
Competing Interests: M. Arreguin-Nava is employed by Eco-Bio LLC. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Databáze: MEDLINE
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