| Autor: |
Scuratti F; Center for Nano Science and Technology @PoliMi, Istituto Italiano di Tecnologia , Via Giovanni Pascoli, 70/3 , 20133 Milano , Italy.; Department of Electronics, Information and Bioengineering , Politecnico di Milano , Piazza Leonardo da Vinci, 32 , 20133 Milano , Italy., Bonacchini GE; Center for Nano Science and Technology @PoliMi, Istituto Italiano di Tecnologia , Via Giovanni Pascoli, 70/3 , 20133 Milano , Italy., Bossio C; Center for Nano Science and Technology @PoliMi, Istituto Italiano di Tecnologia , Via Giovanni Pascoli, 70/3 , 20133 Milano , Italy., Salazar-Rios JM; Zernike Institute for Advanced Materials, University of Groningen , Nijenborgh 4 9747 AG Groningen , The Netherlands., Talsma W; Zernike Institute for Advanced Materials, University of Groningen , Nijenborgh 4 9747 AG Groningen , The Netherlands., Loi MA; Zernike Institute for Advanced Materials, University of Groningen , Nijenborgh 4 9747 AG Groningen , The Netherlands., Antognazza MR; Center for Nano Science and Technology @PoliMi, Istituto Italiano di Tecnologia , Via Giovanni Pascoli, 70/3 , 20133 Milano , Italy., Caironi M; Center for Nano Science and Technology @PoliMi, Istituto Italiano di Tecnologia , Via Giovanni Pascoli, 70/3 , 20133 Milano , Italy. |
| Abstrakt: |
Cell-based biosensors constitute a fundamental tool in biotechnology, and their relevance has greatly increased in recent years as a result of a surging demand for reduced animal testing and for high-throughput and cost-effective in vitro screening platforms dedicated to environmental and biomedical diagnostics, drug development, and toxicology. In this context, electrochemical/electronic cell-based biosensors represent a promising class of devices that enable long-term and real-time monitoring of cell physiology in a noninvasive and label-free fashion, with a remarkable potential for process automation and parallelization. Common limitations of this class of devices at large include the need for substrate surface modification strategies to ensure cell adhesion and immobilization, limited compatibility with complementary optical cell-probing techniques, and the need for frequency-dependent measurements, which rely on elaborated equivalent electrical circuit models for data analysis and interpretation. We hereby demonstrate the monitoring of cell adhesion and detachment through the time-dependent variations in the quasi-static characteristic current curves of a highly stable electrolyte-gated transistor, based on an optically transparent network of printable polymer-wrapped semiconducting carbon-nanotubes. |
