Splenic ischemic preconditioning attenuates oxidative stress induced by hepatic ischemia-reperfusion in rats.

Autor:	Costa CCC; Graduate student, Faculty of Medicine, UEPA, Belem-PA, Brazil. Technical procedures, analysis and interpretation of data, manuscript preparation., Pereira NG; Graduate student, Faculty of Medicine, UEPA, Belem-PA, Brazil. Technical procedures, analysis and interpretation of data, manuscript preparation., Machado ALM; Graduate student, Faculty of Medicine, UEPA, Belem-PA, Brazil. Technical procedures, analysis and interpretation of data, manuscript preparation., Dórea MA; Graduate student, Faculty of Medicine, UEPA, Belem-PA, Brazil. Technical procedures, analysis and interpretation of data, manuscript preparation., Cruz RMMD; Graduate student, Faculty of Medicine, UEPA, Belem-PA, Brazil. Technical procedures, analysis and interpretation of data, manuscript preparation., Silva RC; Fellow, Postgraduate Program in Surgery and Experimental Research, UEPA, Belem-PA, Brazil. Technical procedures, analysis and interpretation of data, manuscript preparation., Domingues RJS; PhD, Full Professor, Department of Morphology and Physiological Sciences, UEPA, Belem-PA, Brazil. Scientific and intellectual content of the study, critical revision, final approval., Yasojima EY; PhD, Full Professor, Postgraduate Program in Surgery and Experimental Research, Universidade do Estado do Pará (UEPA), Belem-PA, Brazil. Conception, design, scientific and intellectual content of the study; critical revision; final approval.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2019 Sep 16; Vol. 34 (7), pp. e201900707. Date of Electronic Publication: 2019 Sep 16.
DOI:	10.1590/s0102-865020190070000007
Abstrakt:	Purpose: To evaluate the effects of splenic ischemic preconditioning (sIPC) on oxidative stress induced by hepatic ischemia-reperfusion in rats. Methods: Fifteen male Wistar rats were equally divided into 3 groups: SHAM, IRI and sIPC. Animals from IRI group were subjected to 45 minutes of partial liver ischemia (70%). In the sIPC group, splenic artery was clamped in 2 cycles of 5 min of ischemia and 5 min of reperfusion (20 min total) prior to hepatic ischemia. SHAM group underwent the same surgical procedures as in the remaining groups, but no liver ischemia or sIPC were induced. After 1h, hepatic and splenic tissue samples were harvested for TBARS, CAT, GPx and GSH-Rd measurement. Results: sIPC treatment significantly decreased both hepatic and splenic levels of TBARS when compared to IRI group (p<0.01). Furthermore, the hepatic and splenic activities of CAT, GPx and GSH- Rd were significantly higher in sIPC group than in IRI group. Conclusion: sIPC was able to attenuate hepatic and splenic IRI-induced oxidative stress.
Databáze:	MEDLINE
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