Granulocyte colony-stimulating factor downregulates interferon-gamma receptor expression and stimulates interleukin-6 production in activated human macrophages.

Autor: Seledtsov VI; Center for Medical Biotechnologies, Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Malashchenko VV; Center for Medical Biotechnologies, Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Meniailo ME; Center for Medical Biotechnologies, Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Gazatova ND; Center for Medical Biotechnologies, Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Seledtsova GV; Laboratory for Cellular Technologies, Institute for Fundamental and Clinical Immunology, Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: Growth factors (Chur, Switzerland) [Growth Factors] 2019 Aug; Vol. 37 (3-4), pp. 164-169. Date of Electronic Publication: 2019 Sep 18.
DOI: 10.1080/08977194.2019.1662418
Abstrakt: We studied direct effects of human granulocyte colony-stimulating factor (G-CSF) on phenotypical properties of human macrophage cells in vitro . CD14 + monocyte/macrophages (Mc/Mphs) were isolated from blood of healthy donors by positive magnetic separation. G-CSF (0.01-1.0 ng/mL), when added to Mc/Mphs along with lipopolysaccharide (LPS, 1.0 μg/mL), was able to noticeably reduce proportions of CD119 (interferon-γ receptor 1)-positive cells, with no stable effects on CD16 (FcγRIII) + and СD124 (IL-4 receptor subunit alpha)-positive cells. In addition, G-CSF markedly upregulated IL-6 production by LPS-activated Mph cells, without significantly affecting IL-1β, IL-10 and tumor necrosis factor-α (TNF-α) secretion. Our data suggests that G-CSF could restrain Mph polarization to pro-inflammatory (M1) phenotype, thus potentially supporting pro-regenerative Mph activity with implications for immunotherapeutic interventions.
Databáze: MEDLINE
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