[The development of immune enzyme test-systems for detecting potentially pandemic influenza viruses subtype A(H2), A(H5), A(H7) and A(H9).]

Autor:	Petrova ER; The research institute of influenza of Minzdrav of Russia, 197376, St. Petersburg, Russia., Krivitskaya VZ; The research institute of influenza of Minzdrav of Russia, 197376, St. Petersburg, Russia., Sorokin EV; The research institute of influenza of Minzdrav of Russia, 197376, St. Petersburg, Russia., Tsareva TR; The research institute of influenza of Minzdrav of Russia, 197376, St. Petersburg, Russia., Maiorova VG; The research institute of influenza of Minzdrav of Russia, 197376, St. Petersburg, Russia., Timoshitcheva TA; The research institute of influenza of Minzdrav of Russia, 197376, St. Petersburg, Russia., Sominina AA; The research institute of influenza of Minzdrav of Russia, 197376, St. Petersburg, Russia.
Jazyk:	ruština
Zdroj:	Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2016; Vol. 61 (7), pp. 432-438.
DOI:	10.18821/0869-2084-2016-61-7-432-438
Abstrakt:	Purpose of Study: To develop diagnostic test-systems constructed according principle of "sandwich" enzyme-linked immunosorbent assay to detect antigens of influenza viruses with pandemic potential: A(H2), A(H5), A(H7), A(H9).The panels of subtype-specific monoclonal antibodies interacting with molecule of hemagglutinin of potentially pandemic viruses of influenza are obtained and characterized. The viruses of every sub-type were supplied with selected pairs monoclonal antibodies/monoclonal antibodies' conjugate with peroxidase of horse-radish, interacting most effectively with virus-immunogen and lacking non-specific activity related to influenza viruses of geterologic types. Considering established threshold values, the sensitivity of test-systems varied depending on sub-type and strain of viruses within limits of 4 - 30 ng/ml at evaluation of viral purified concentrates and 0.3 - 8 hemagglutinin units at analysis of virus-containing allantoic fluid. The specificity of immune-enzyme test-system manifested in conditions of absence of non-specific interactions with both seasonal and potentially pandemic geterologic viruses. The technique can be applied for fast identification of viruses of influenza non-typeable in conditions of routine functioning of practical laboratories. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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