Lack of synaptic protein, calsyntenin-2, impairs morphology of synaptic complexes in mice.

Autor: Ranneva SV; Federal State Budgetary Scientific Institution, Scientific Research Institute of Physiology and Basic Medicine, Novosibirsk, Russia., Maksimov VF; Federal State Budgetary Scientific Institution, Scientific Research Institute of Physiology and Basic Medicine, Novosibirsk, Russia., Korostyshevskaja IM; Federal State Budgetary Scientific Institution, Scientific Research Institute of Physiology and Basic Medicine, Novosibirsk, Russia., Lipina TV; Federal State Budgetary Scientific Institution, Scientific Research Institute of Physiology and Basic Medicine, Novosibirsk, Russia.; Department of Medicine and Psychology, Novosibirsk State University, Novosibirsk, Russia.; Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada.
Jazyk: angličtina
Zdroj: Synapse (New York, N.Y.) [Synapse] 2020 Feb; Vol. 74 (2), pp. e22132. Date of Electronic Publication: 2019 Sep 24.
DOI: 10.1002/syn.22132
Abstrakt: Calsyntenin-2 (Clstn2) is the synaptic protein, which belongs to the superfamily of cadherins, playing an important role in learning and memory. We recently reported that Clstn2 knockout mice (Clstn2-KO) have a deficit of GABAergic interneurons, associated with hyperactivity, deficient spatial memory, and social behavior. Therefore, we sought to characterize morphometric features of the ultrastructure of synaptic complexes of hippocampal and cortical neurons in Clstn2-KO mice, using high magnification electron microscopy. Morphometric analysis revealed a reduction of symmetric (inhibitory) synaptic density, length of synaptic contacts, and postsynaptic density in neurons of Clstn2-KO mice. Moreover, cortical neurons of Clstn2-KO mice were characterized by the predominance of the simplified type of synapses with the emergence of negative curvature of the synaptic zone in Clstn2-KO mice. Notably, presynaptic zones of cortical neurons of Clstn2-KO mice were characterized by the increased number of synaptic vesicles in opposite to the decreased number of synaptic vesicles in the presynaptic zones of hippocampal neurons. Overall, we found that lack of calsyntenin-2 leads to the striking architectonic alterations of synaptic complexes in the mouse brain, disrupting synaptic density, shape, and connectivity.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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