Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India.

Autor: Nagarajan G; Southern Regional Research Centre, ICAR-Central Sheep and Wool Research Institute, Kodaikanal, Tamil Nadu, India., Pourouchottamane R; ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh, India., Reddy GBM; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, India., Yogisharadhya R; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, India., Sumana K; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, India., Rajapandi S; ICAR-Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India., Murali G; Southern Regional Research Centre, ICAR-Central Sheep and Wool Research Institute, Kodaikanal, Tamil Nadu, India., Thirumaran SMK; Southern Regional Research Centre, ICAR-Central Sheep and Wool Research Institute, Kodaikanal, Tamil Nadu, India., Mallick PK; ICAR-Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India., Rajendiran AS; Southern Regional Research Centre, ICAR-Central Sheep and Wool Research Institute, Kodaikanal, Tamil Nadu, India.
Jazyk: angličtina
Zdroj: Veterinary world [Vet World] 2019 Jul; Vol. 12 (7), pp. 1022-1027. Date of Electronic Publication: 2019 Jul 12.
DOI: 10.14202/vetworld.2019.1022-1027
Abstrakt: Aim: The present study was carried out to find out the causative agent of exanthematous skin lesions in sheep maintained by Southern Regional Research Centre, Mannavanur, Kodai hills, Tamil Nadu.
Materials and Methods: Polymerase chain reaction (PCR) with Orf virus (ORFV) B2L gene-specific primers was carried out by employing the total genomic DNA isolated from the scabs as the template. The ORFV isolates from Kodai hills were characterized by the use of bioinformatics tools.
Results: The amino acid identity of ORFV isolate 1 from Kodai hills is having 98.14%, 96.29%, and 83.59% identity with reference strains of ORFV, Pseudocowpox virus, and bovine papular stomatitis virus, respectively. Phylogenetic analysis revealed that ORFV isolates from Kodai hills clustered with the other ORFV isolates from different geographical areas of India.
Conclusion: The etiological agent of exanthematous skin lesion among sheep of Kodai hills is ORFV.
Databáze: MEDLINE
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