| Autor: |
Galermo AG; Department of Chemistry , University of California Davis , Davis , California 95616 , United States., Nandita E; Department of Chemistry , University of California Davis , Davis , California 95616 , United States., Castillo JJ; Department of Chemistry , University of California Davis , Davis , California 95616 , United States., Amicucci MJ; Department of Chemistry , University of California Davis , Davis , California 95616 , United States.; Agricultural and Environmental Chemistry Graduate Group , University of California Davis , Davis , California 95616 , United States., Lebrilla CB; Department of Chemistry , University of California Davis , Davis , California 95616 , United States. |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical chemistry [Anal Chem] 2019 Oct 15; Vol. 91 (20), pp. 13022-13031. Date of Electronic Publication: 2019 Sep 30. |
| DOI: |
10.1021/acs.analchem.9b03101 |
| Abstrakt: |
The extensive characterization of glycosidic linkages in carbohydrates remains a challenge because of the lack of known standards and limitations in current analytical techniques. This study encompasses the construction of an extensive glycosidic linkage library built from synthesized standards. It includes an improved liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantitation of glycosidic linkages derived from disaccharides, oligosaccharides, and polysaccharides present in complicated matrices. We present a method capable of the simultaneous identification of over 90 unique glycosidic linkages using ultrahigh-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC/QqQ MS) operated in dynamic multiple reaction monitoring (dMRM) mode. To build the library, known monosaccharides commonly found in plants were subjected to partial methylation to yield partially derivatized species representing trisecting, bisecting, linear, and terminal structures. The library includes glycosidic linkage information for three hexoses (glucose, galactose, and mannose), three pentoses (xylose, arabinose, and ribose), two deoxyhexoses (fucose and rhamnose), and two hexuronic acids (glucuronic acid and galacturonic acid). The resulting partially methylated monosaccharides were then labeled with 1-phenyl-3-methyl-5-pyrazolone (PMP) followed by separation and analysis by UHPLC/dMRM MS. Validation of the synthesized standards was performed using disaccharide, oligosaccharide, and polysaccharide standards. Accuracy, reproducibility, and robustness of the method was demonstrated by analysis of xyloglucan (tamarind) and whole carrot root. The synthesized standards represent the most comprehensive group of carbohydrate linkages to date. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
