Short-term aromatase inhibition induces prostatic alterations in adult wistar rat: A biochemical, histopathological and immunohistochemical study.

Autor: Cheboub A; Faculty of Biology Sciences, University of Sciences and Technology Houari Boumediene, Algeria. Electronic address: acheboub@usthb.dz., Regouat N; Faculty of Biology Sciences, University of Sciences and Technology Houari Boumediene, Algeria., Djidjik R; Immunology Service of Isaad Hassani-Beni Messous Hospital, Algiers, Algeria., Slimani A; Pathological Anatomy Service of Isaad Hassani-Beni Messous Hospital, Algiers, Algeria., Hadj-Bekkouche F; Faculty of Biology Sciences, University of Sciences and Technology Houari Boumediene, Algeria.
Jazyk: angličtina
Zdroj: Acta histochemica [Acta Histochem] 2019 Nov; Vol. 121 (8), pp. 151441. Date of Electronic Publication: 2019 Sep 12.
DOI: 10.1016/j.acthis.2019.151441
Abstrakt: Purpose: This study aimed to evaluate the effects of estrogen reduction on amyloid deposition, some lipid metabolism and oxidative stress markers, PSA-like production and p63 expression in the prostate of the adult rat.
Methods: Aromatase inhibitor: Formestane (4-OHA), was administrated to male rats, at a dose of 0.1 mg/kg b.w./day, for 10 days. The control group (CONT) received the same volume of placebo injection (NaCl 0.9%).
Results: 4-OHA treatment induced a significant accumulation of intraprostatic cholesterol (138.90 ± 17.64 vs 85.12 ± 2.87, p = 0.01); against an insignificant diminution of malondialdehyde (412.6 ± 54.35 vs 842.70 ± 336.50, p > 0.05) and glutathione (2.40 ± 0.23 vs 3.65 ± 0.88, p > 0.05). This was associated with a significant decrease of nitric oxide (31.76 ± 7.07 vs 179.40 ± 58.35, p = 0.024). Additionally, 4-OHA significantly increased the intraprostatic production of PSA-like (11.12 ± 2.78 vs 3.91 ± 0.43, p = 0.043). The prostatic histology revealed an amyloid deposition, in all prostatic lobes and a smooth muscle layer growth (p < 0.05); especially significant in the dorsal and lateral lobes. Theses lobes manifested a basal cells proliferation, with a 3-fold increase of p63 expression (p < 0.001). The ventral lobe presented epithelial atrophy (37.80 ± 16.20 vs 167.60 ± 5.16, p < 0.05); with occasional and significant proliferative foci (247.00 ± 9.573 vs 167.60 ± 5.16 p < 0.05).
Discussion and Conclusion: Aromatase inhibition, in the adult male rat, alters the prostatic function by reducing nitric oxide availability and inducing amyloid deposition along with limiting the differentiation of basal cells, through a lobe-specific p63-overexpression.
(Copyright © 2019 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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