Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies.

Autor: Maciel MODS; Department of Clinic, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University, Araçatuba, SP, Brazil., Soares MF; Department of Clinic, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University, Araçatuba, SP, Brazil., Costa SF; Department of Clinic, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University, Araçatuba, SP, Brazil., Bragato JP; Department of Clinic, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University, Araçatuba, SP, Brazil., de Freitas JH; Department of Clinic, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University, Araçatuba, SP, Brazil., Venturin GL; Department of Clinic, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University, Araçatuba, SP, Brazil., Melo LM; Department of Clinic, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University, Araçatuba, SP, Brazil., Rebech GT; Department of Clinic, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University, Araçatuba, SP, Brazil., Reed S; Infectious Disease Research Institute, Seattle, WA, USA., Lima VMF; Department of Clinic, Surgery and Animal Reproduction, School of Veterinary Medicine, São Paulo State University, Araçatuba, SP, Brazil. Electronic address: vmflima@fmva.unesp.br.
Jazyk: angličtina
Zdroj: Journal of immunological methods [J Immunol Methods] 2019 Nov; Vol. 474, pp. 112664. Date of Electronic Publication: 2019 Sep 12.
DOI: 10.1016/j.jim.2019.112664
Abstrakt: Recently, a novel Enzyme-Linked Immunosorbent Assay (ELISA) strategy has emerged, known as "plasmonic ELISA" (pELISA), which enables the detection of disease biomarkers at low concentrations with the naked eye. For the first time, this research has developed a signal-generation mechanism for the detection of anti-Leishmania sp. IgG antibodies with the naked eye using pELISA. The immunoassay incorporates an indirect ELISA with successive growth of gold nanoparticles to obtain blue or red-colored solutions in the presence or absence of anti-Leishmania sp. IgG antibodies in canine serum, respectively. The technique we developed was successfully tested in canine serum positive and negative for canine leishmaniasis (CanL), and was shown to be an effective method that could be used as an additional tool for CanL diagnosis. It will be particularly useful in resource-constrained countries, because it does not require sophisticated instruments to read the results, increasing the practicality of CanL detection in these areas.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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