Carbohydrate-independent antibiofilm effect of Bothrops jararacussu lectin BJcuL on Staphylococcus aureus.

Autor: Aguilar AP; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Minas Gerais, Brazil., Onofre TS; Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil., Fabres-Klein MH; Centro de Ciências Biológicas e da Saúde, Universidade Federal do Oeste da Bahia, Bahia, Brazil., Klein RC; Centro de Ciências Biológicas e da Saúde, Universidade Federal do Oeste da Bahia, Bahia, Brazil., Feio RN; Museu de Zoologia João Moojen, Departamento de Biologia Animal, Universidade Federal de Viçosa, Minas Gerais, Brazil., de Oliveira Mendes TA; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Minas Gerais, Brazil., de Oliveira Barros Ribon A; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Minas Gerais, Brazil. Electronic address: abribon@ufv.br.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2019 Dec; Vol. 137, pp. 103745. Date of Electronic Publication: 2019 Sep 11.
DOI: 10.1016/j.micpath.2019.103745
Abstrakt: The antivirulence approach to fighting biofilm-based infections caused by Staphylococcus aureus is a promising therapy that has been studied extensively. Here, we compare the antibiofilm activity of a purified lectin from Bothrops jararacussu venom (BJcuL) and commercial lectins obtained from Triticum vulgaris (Wheat Germ Agglutinin, WGA), Bandeiraea simplicifolia BS-II, and Maclura pomifera. Only WGA had antibiofilm activity, although no effect was seen on pre-formed biofilms. The pre-incubation of WGA and BJcuL with their preferential sugars inhibited the biological activity of WGA, but not that of BJcuL, suggesting that biofilm disruption does not involve carbohydrate-recognition domains (CRDs). Quantitative real-time PCR showed that BJcuL promotes modulation of expression of S. aureus genes involved in biofilm formation. Light microscopy revealed cocci and small cell clusters after biofilm formation in the presence of BJcuL, showing that the lectin treatment was unable to completely disrupt biofilm structure. Exposing the free cells to 50 times the minimum inhibitory concentration of gentamicin or ciprofloxacin did not prevent biofilm reestablishment, although inhibition was stronger than in the control (no lectin). This disruption of the biofilm architecture can expose the bacterial cell and may facilitate clearance by the immune system.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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