Leishmania infantum-derived lipophosphoglycan as an antigen in the accurate serodiagnosis of canine leishmaniasis.

Autor: Portela RD; Laboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia (UFBA); Salvador, Bahia; Brazil., Soares RP; Laboratory of Cellular and Molecular Parasitology, René Rachou Institute, Oswaldo Cruz Foundation; Belo Horizonte, Minas Gerais; Brazil., Passos GP; Laboratory of Veterinary Infectious Diseases, Teaching Hospital of Veterinary Medicine, UFBA; Salvador, Bahia; Brazil., Larangeira DF; Laboratory of Veterinary Infectious Diseases, Teaching Hospital of Veterinary Medicine, UFBA; Salvador, Bahia; Brazil.; Department of Anatomy, Pathology, and Veterinary Clinics of the School of Veterinary Medicine and Zootechny, UFBA, Salvador, BA, Brazil, CEP., Barral TD; Laboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia (UFBA); Salvador, Bahia; Brazil., Sampaio JR; Laboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia (UFBA); Salvador, Bahia; Brazil., Bernardo MF; Laboratory of Immunology and Molecular Biology, Institute of Health Sciences, Federal University of Bahia (UFBA); Salvador, Bahia; Brazil., Alves-Sobrinho EV; Laboratory of Cellular and Molecular Parasitology, René Rachou Institute, Oswaldo Cruz Foundation; Belo Horizonte, Minas Gerais; Brazil., Bahia MT; Institute of Exact and Biological Sciences, Federal University of Ouro Preto, Campus Morro do Cruzeiro; Ouro Preto, Minas Gerais; Brazil., Pinho FA; Laboratory of Veterinary Infectious Diseases, Teaching Hospital of Veterinary Medicine, UFBA; Salvador, Bahia; Brazil.; Department of Anatomy, Pathology, and Veterinary Clinics of the School of Veterinary Medicine and Zootechny, UFBA, Salvador, BA, Brazil, CEP., Barrouin-Melo SM; Laboratory of Veterinary Infectious Diseases, Teaching Hospital of Veterinary Medicine, UFBA; Salvador, Bahia; Brazil.; Department of Anatomy, Pathology, and Veterinary Clinics of the School of Veterinary Medicine and Zootechny, UFBA, Salvador, BA, Brazil, CEP.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2019 Sep 12; Vol. 13 (9), pp. e0007720. Date of Electronic Publication: 2019 Sep 12 (Print Publication: 2019).
DOI: 10.1371/journal.pntd.0007720
Abstrakt: Lipophosphoglycan (LPG) is the major surface glycoconjugate of Leishmania protozoan and has an important biological role in host-parasite interactions both in the midgut epithelium of the sand fly vector and in the vertebrate macrophages. Canine leishmaniasis (CanL) is a chronic infectious disease predominantly caused by Leishmania infantum. An early and accurate immunodiagnosis of the disease is crucial for veterinary clinical practice and for disease control. In this work, we evaluated L. infantum LPG as an antigen in an indirect enzyme-linked immunosorbent assay (ELISA) for CanL immunodiagnosis (LPG-ELISA) by testing serum samples from 97 naturally infected dogs with diverse clinical presentations ranging from subclinical infection to severe disease, as evaluated by veterinarian infectologists. Serum samples from healthy dogs from non-endemic areas (n = 68) and from dogs with other infectious diseases (n = 64) were used as controls for assay validation. The performance of the LPG-ELISA was compared with that of an ELISA using the soluble fraction of L. infantum total lysate antigen (TLA). LPG-ELISA presented a superior performance in comparison to TLA-ELISA, with 91.5% sensitivity, 98.5% specificity and 99.7% accuracy. A distinguishing feature of the LPG-ELISA compared to the TLA-ELISA was its higher ability to identify subclinical infection in clinically healthy dogs, in addition to the absence of cross-reactivity with other canine infectious diseases. Finally, LPG-ELISA was compared to TR DPP visceral canine leishmaniasis test, the immunochromatographic test recommended by the Brazilian Ministry of Agriculture. LPG-ELISA exhibited higher values of specificity (98.5% versus 93.1%) and sensitivity (91.5% versus 90.6%) compared to TR DPP. In conclusion, L. infantum-derived LPG was recognized by antibodies elicited during CanL in different infection stages and was shown to be a suitable antigen for specific clinical settings of veterinary diagnosis and for public health usage.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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