Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus.
| Autor: | Draper JM; Transgenic and Gene-Targeting Institutional Facility, University of Kansas Medical Center, Kansas City, KS, USA., Vivian JL; Transgenic and Gene-Targeting Institutional Facility, University of Kansas Medical Center, Kansas City, KS, USA. jvivian@kumc.edu.; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA. jvivian@kumc.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2066, pp. 217-234. |
| DOI: | 10.1007/978-1-4939-9837-1_18 |
| Abstrakt: | Induced pluripotent stem (iPS) cells are important tools for studying differentiation and for use in patient-specific disease modeling. We present a detailed method for the reprogramming of primary human fibroblasts to induced pluripotent stem cells using Sendai virus. These procedures allow for the efficient generation of multiple high-quality feeder-independent iPS cell lines for a given human fibroblast line. The iPS cell lines generated by this protocol can be used in a variety of differentiation and gene expression studies, as well as in genetic manipulations. |
| Databáze: | MEDLINE |
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