A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum.

Autor: Schindler T; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland. tobias.schindler@unibas.ch.; University of Basel, Basel, Switzerland. tobias.schindler@unibas.ch., Deal AC; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.; University of Basel, Basel, Switzerland., Fink M; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.; University of Basel, Basel, Switzerland., Guirou E; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.; University of Basel, Basel, Switzerland., Moser KA; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA., Mwakasungula SM; Bagamoyo Research and Training Centre, Ifakara Health Institute, Bagamoyo, United Republic of Tanzania., Mihayo MG; Bagamoyo Research and Training Centre, Ifakara Health Institute, Bagamoyo, United Republic of Tanzania., Jongo SA; Bagamoyo Research and Training Centre, Ifakara Health Institute, Bagamoyo, United Republic of Tanzania., Chaki PP; Bagamoyo Research and Training Centre, Ifakara Health Institute, Bagamoyo, United Republic of Tanzania., Abdulla S; Bagamoyo Research and Training Centre, Ifakara Health Institute, Bagamoyo, United Republic of Tanzania., Valverde PCM; Laboratorios de Investigacion y Desarrollo, Facultad de Ciencias y Filosofia & Instituto de Medicina Tropical, Alexander von Humboldt Universidad Peruana Cayetano Heredia, Lima, Peru., Torres K; Laboratorios de Investigacion y Desarrollo, Facultad de Ciencias y Filosofia & Instituto de Medicina Tropical, Alexander von Humboldt Universidad Peruana Cayetano Heredia, Lima, Peru., Bijeri JR; Equatorial Guinea Malaria Vaccine Initiative, Malabo, Equatorial Guinea., Silva JC; Laboratorios de Investigacion y Desarrollo, Facultad de Ciencias y Filosofia & Instituto de Medicina Tropical, Alexander von Humboldt Universidad Peruana Cayetano Heredia, Lima, Peru., Hoffman SL; Sanaria Inc., Rockville, Maryland, USA., Gamboa D; Laboratorios de Investigacion y Desarrollo, Facultad de Ciencias y Filosofia & Instituto de Medicina Tropical, Alexander von Humboldt Universidad Peruana Cayetano Heredia, Lima, Peru., Tanner M; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.; University of Basel, Basel, Switzerland., Daubenberger C; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland. claudia.daubenberger@swisstph.ch.; University of Basel, Basel, Switzerland. claudia.daubenberger@swisstph.ch.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Sep 11; Vol. 9 (1), pp. 13107. Date of Electronic Publication: 2019 Sep 11.
DOI: 10.1038/s41598-019-49389-2
Abstrakt: The rapid and accurate diagnosis of Plasmodium falciparum malaria infection is an essential factor in malaria control. Currently, malaria diagnosis in the field depends heavily on using rapid diagnostic tests (RDTs) many of which detect circulating parasite-derived histidine-rich protein 2 antigen (PfHRP2) in capillary blood. P. falciparum strains lacking PfHRP2, due to pfhrp2 gene deletions, are an emerging threat to malaria control programs. The novel assay described here, named qHRP2/3-del, is well suited for high-throughput screening of P. falciparum isolates to identify these gene deletions. The qHRP2/3-del assay identified pfhrp2 and pfhrp3 deletion status correctly in 93.4% of samples with parasitemia levels higher than 5 parasites/µL when compared to nested PCR. The qHRP2/3-del assay can correctly identify pfhrp2 and pfhrp3 gene deletions in multiple strain co-infections, particularly prevalent in Sub-Saharan countries. Deployment of this qHRP2/3-del assay will provide rapid insight into the prevalence and potential spread of P. falciparum isolates that escape surveillance by RDTs.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje