| Autor: |
Kolling J; Programa de Pós-Graduação em Nanociências, Universidade Franciscana/UFN, Santa Maria, RS, Brazil., Kolling J; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul/UFRGS, Porto Alegre, RS, Brazil., Franceschi ID; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul/UFRGS, Porto Alegre, RS, Brazil., Nishihira VSK; Programa de Pós-Graduação em Nanociências, Universidade Franciscana/UFN, Santa Maria, RS, Brazil., Baldissera MD; Programa de Pós-Graduação em Nanociências, Universidade Franciscana/UFN, Santa Maria, RS, Brazil., Pinto CG; Programa de Pós-Graduação em Nanociências, Universidade Franciscana/UFN, Santa Maria, RS, Brazil., Mezzomo NJ; Programa de Pós-Graduação em Nanociências, Universidade Franciscana/UFN, Santa Maria, RS, Brazil., Carmo GMD; Programa de Pós-Graduação em Nanociências, Universidade Franciscana/UFN, Santa Maria, RS, Brazil., Feksa LR; Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, RS, Brazil., Fernandes LS; Programa de Pós-Graduação em Nanociências, Universidade Franciscana/UFN, Santa Maria, RS, Brazil., Orengo G; Programa de Pós-Graduação em Nanociências, Universidade Franciscana/UFN, Santa Maria, RS, Brazil., Vaucher RA; Programa de Pós-Graduação em Bioquímica e Bioprospecção, Universidade Federal de Pelotas/UFPel, Pelotas, RS, Brazil., Giongo JL; Universidade Integrada do Alto Uruguai/URI, Santiago, RS, Brazil., Wyse ATS; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul/UFRGS, Porto Alegre, RS, Brazil., Wannmacher CMD; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul/UFRGS, Porto Alegre, RS, Brazil., Rech VC; Programa de Pós-Graduação em Nanociências, Universidade Franciscana/UFN, Santa Maria, RS, Brazil. |
| Abstrakt: |
Type 1 diabetes (T1D) is the result of the selective destruction of the pancreatic β-cells by T cells of the immune system. Although spleen is a secondary lymphoid organ, it is also involved in the T1D pathogenesis. However, the alterations in a variety of cellular processes of this disease need to be further understood. We aimed to analyze the benefits of resveratrol, and its complexed form on diabetic complications in the spleen of rats. To this end, we investigated important enzymes of phosphoryl transfer network, and Na+, K+-ATPase activity. Wistar rats were divided into non-diabetic groups: Control, Ethanol, Resveratrol, Hydroxypropyl-β-cyclodextrin, Resveratrol-hydroxypropyl-β-cyclodextrin, and diabetic groups with the same treatments. Diabetes was induced by a single dose of 60 mg/kg of streptozocin intraperitoneally, and treatments by intragastric gavage once daily for 60 days. Hyperglycemia reduced creatine kinase activity, which was reversed by the administration of resveratrol. Na+, K+-ATPase activity was greatly affected, but it was reversed by resveratrol and resveratrol-hydroxypropyl-β-cyclodextrin. This suggest an energetic imbalance in the spleen of diabetic rats, and in case this also occurs in the diabetic patients, it is possible that resveratrol supplementation could be beneficial to the better functioning of the spleen in diabetic patients. |
