| Autor: |
Meital LT; Centre for Genetics, Ecology & Physiology, School of Health and Sport Sciences, University of the Sunshine Coast, Sippy Downs, Qld, Australia.; VasoActive Group, School of Health and Sport Sciences, University of the Sunshine Coast, Sippy Downs, Qld, Australia., Windsor MT; VasoActive Group, School of Health and Sport Sciences, University of the Sunshine Coast, Sippy Downs, Qld, Australia., Perissiou M; VasoActive Group, School of Health and Sport Sciences, University of the Sunshine Coast, Sippy Downs, Qld, Australia., Schulze K; Sunshine Vascular, Buderim, Qld, Australia., Magee R; Sunshine Coast University Hospital, Birtinya, Qld, Australia., Kuballa A; Centre for Genetics, Ecology & Physiology, School of Health and Sport Sciences, University of the Sunshine Coast, Sippy Downs, Qld, Australia., Golledge J; Queensland Research Centre for Peripheral Vascular Disease, College of Medicine and Dentistry, James Cook University, Townsville, Australia.; Department of Vascular and Endovascular Surgery, Townsville Hospital, Townsville, Australia., Bailey TG; VasoActive Group, School of Health and Sport Sciences, University of the Sunshine Coast, Sippy Downs, Qld, Australia.; Centre for Research on Exercise, Physical Activity and Health, School of Human Movement and Nutrition Sciences, The University of Queensland, St. Lucia, Qld, Australia., Askew CD; VasoActive Group, School of Health and Sport Sciences, University of the Sunshine Coast, Sippy Downs, Qld, Australia., Russell FD; Centre for Genetics, Ecology & Physiology, School of Health and Sport Sciences, University of the Sunshine Coast, Sippy Downs, Qld, Australia. frussell@usc.edu.au.; VasoActive Group, School of Health and Sport Sciences, University of the Sunshine Coast, Sippy Downs, Qld, Australia. frussell@usc.edu.au. |
| Abstrakt: |
Abdominal aortic aneurysm (AAA) is associated with inflammation and oxidative stress, the latter of which contributes to activation of macrophages, a prominent cell type in AAA. Omega-3 polyunsaturated fatty acids (n-3 PUFAs) have been reported to limit oxidative stress in animal models of AAA. The aim of this study was to evaluate the effect of the n-3 PUFA docosahexaenoic acid (DHA) on antioxidant defence in macrophages from patients with AAA. Cells were obtained from men with small AAA (diameter 3.0-4.5 cm, 75 ± 6 yr, n = 19) and age- matched male controls (72 ± 5 yr, n = 41) and incubated with DHA for 1 h before exposure to 0.1 µg/mL lipopolysaccharide (LPS) for 24 h. DHA supplementation decreased the concentration of tumour necrosis factor-α (TNF-α; control, 42.1 ± 13.6 to 5.1 ± 2.1 pg/ml, p < 0.01; AAA, 25.2 ± 9.8 to 1.9 ± 0.9 pg/ml, p < 0.01) and interleukin-6 (IL-6; control, 44.9 ± 7.7 to 5.9 ± 2.0 pg/ml, p < 0.001; AAA, 24.3 ± 5.2 to 0.5 ± 0.3 pg/ml, p < 0.001) in macrophage supernatants. DHA increased glutathione peroxidase activity (control, 3.2 ± 0.3 to 4.1 ± 0.2 nmol/min/ml/μg protein, p = 0.004; AAA, 2.3 ± 0.5 to 3.4 ± 0.5 nmol/min/ml/μg protein, p = 0.008) and heme oxygenase-1 mRNA expression (control, 1.5-fold increase, p < 0.001). The improvements in macrophage oxidative stress status serve as a stimulus for further investigation of DHA in patients with AAA. |
