A protocol for isolation and culture of mesenchymal stem cells from human gingival tissue.

Autor: Zhang X; Department of Clinical Immunology, The Third Affiliated Hospital at The Sun Yat-sen University Guangzhou 510630, China.; Department of Internal Medicine, The Ohio State University Wexner Medical Center Columbus 43210, Ohio, United States., Zeng D; Department of Clinical Immunology, The Third Affiliated Hospital at The Sun Yat-sen University Guangzhou 510630, China., Huang F; Department of Clinical Immunology, The Third Affiliated Hospital at The Sun Yat-sen University Guangzhou 510630, China., Wang J; Department of Internal Medicine, The Ohio State University Wexner Medical Center Columbus 43210, Ohio, United States.
Jazyk: angličtina
Zdroj: American journal of clinical and experimental immunology [Am J Clin Exp Immunol] 2019 Aug 15; Vol. 8 (4), pp. 21-26. Date of Electronic Publication: 2019 Aug 15 (Print Publication: 2019).
Abstrakt: Human gingiva-derived mesenchymal stem cells (GMSCs) have been considered to be a better source of MSCs for cell therapy in some immunological diseases. We describe a protocol for isolation and culture of mesenchymal stem cells (MSCs) from human gingival tissue in detail, which provides a methodology to help clinical researches and clinical trial. GMSCs are generally isolated from a remnant or discarded tissue following a routine dental procedure, then cultured in complete culture medium at 37°C in a humidified tissue culture incubator with 5% CO 2 and 95% O 2 . Non-adherent cells are removed after 48~72 h and the fresh medium is replaced. When primary cultures become 80%~90% confluent, the plastic-adherent cells are treated with 0.25% trypsin-EDTA and subcultured. A purified population of GMSCs can be obtained 2-3 weeks after the initiation of culture.
Competing Interests: None.
Databáze: MEDLINE
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