Donor-to-Donor Heterogeneity in the Clonal Dynamics of Transplanted Human Cord Blood Stem Cells in Murine Xenografts.

Autor: Belderbos ME; Department of Stem Cell Biology and Ageing, European Research Institute for the Biology of Ageing, University of Groningen, Groningen, Netherlands; Princess Máxima Center for Pediatric Oncology and Oncode Institute, Utrecht, Netherlands. Electronic address: m.e.belderbos@prinsesmaximacentrum.nl., Jacobs S; Department of Stem Cell Biology and Ageing, European Research Institute for the Biology of Ageing, University of Groningen, Groningen, Netherlands., Koster TK; Department of Stem Cell Biology and Ageing, European Research Institute for the Biology of Ageing, University of Groningen, Groningen, Netherlands., Ausema A; Department of Stem Cell Biology and Ageing, European Research Institute for the Biology of Ageing, University of Groningen, Groningen, Netherlands., Weersing E; Department of Stem Cell Biology and Ageing, European Research Institute for the Biology of Ageing, University of Groningen, Groningen, Netherlands., Zwart E; Department of Stem Cell Biology and Ageing, European Research Institute for the Biology of Ageing, University of Groningen, Groningen, Netherlands., de Haan G; Department of Stem Cell Biology and Ageing, European Research Institute for the Biology of Ageing, University of Groningen, Groningen, Netherlands., Bystrykh LV; Department of Stem Cell Biology and Ageing, European Research Institute for the Biology of Ageing, University of Groningen, Groningen, Netherlands.
Jazyk: angličtina
Zdroj: Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation [Biol Blood Marrow Transplant] 2020 Jan; Vol. 26 (1), pp. 16-25. Date of Electronic Publication: 2019 Sep 05.
DOI: 10.1016/j.bbmt.2019.08.026
Abstrakt: Umbilical cord blood (UCB) provides an alternative source of hematopoietic stem cells (HSCs) for allogeneic transplantation. Administration of sufficient donor HSCs is critical to restore recipient hematopoiesis and to maintain long-term polyclonal blood formation. However, due to lack of unique markers, the frequency of HSCs among UCB CD34 + cells is the subject of ongoing debate, urging for reproducible strategies for their counting. Here, we used cellular barcoding to determine the frequency and clonal dynamics of human UCB HSCs and to determine how data analysis methods affect these parameters. We transplanted lentivirally barcoded CD34 + cells from 20 UCB donors into Nod/Scid/IL2Ry -/- (NSG) mice (n = 30). Twelve recipients (of 8 UCB donors) engrafted with >1% GFP + cells, allowing for clonal analysis by multiplexed barcode deep sequencing. Using multiple definitions of clonal diversity and strategies for data filtering, we demonstrate that differences in data analysis can change clonal counts by several orders of magnitude and propose methods to improve their consistency. Using these methods, we show that the frequency of NSG-repopulating cells was low (median ∼1 HSC/10 4 CD34 + UCB cells) and could vary up to 10-fold between donors. Clonal patterns in blood became increasingly consistent over time, likely reflecting initial output of transient progenitors, followed by long-term HSCs with stable hierarchies. The majority of long-term clones displayed multilineage output, yet clones with lymphoid- or myeloid-biased output were also observed. Altogether, this study uncovers substantial interdonor and analysis-induced variability in the frequency of UCB CD34 + clones that contribute to post-transplant hematopoiesis. As clone tracing is increasingly relevant, we urge for universal and transparent methods to count HSC clones during normal aging and upon transplantation.
(Copyright © 2019 American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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