Anti-oxidative function of follicular fluid HDL and outcomes of modified natural cycle-IVF.

Autor: Nagy RA; Department of Paediatrics, Centre for Liver, Digestive, and Metabolic Diseases, University of Groningen, University Medical Centre Groningen, 9713 GZ, Groningen, The Netherlands.; Department of Obstetrics and Gynaecology, Section Reproductive Medicine, University of Groningen, University Medical Centre Groningen, 9713 GZ, Groningen, The Netherlands., van Montfoort APA; Department of Obstetrics and Gynaecology, Section Reproductive Medicine, University of Groningen, University Medical Centre Groningen, 9713 GZ, Groningen, The Netherlands.; Department of Obstetrics & Gynaecology, GROW School for Oncology and Developmental Biology, Maastricht University Medical Centre, 6229 HX, Maastricht, The Netherlands., Groen H; Department of Epidemiology, University of Groningen, University Medical Centre Groningen, 9713 GZ, Groningen, The Netherlands., Homminga I; Department of Obstetrics and Gynaecology, Section Reproductive Medicine, University of Groningen, University Medical Centre Groningen, 9713 GZ, Groningen, The Netherlands., Andrei D; Department of Paediatrics, Centre for Liver, Digestive, and Metabolic Diseases, University of Groningen, University Medical Centre Groningen, 9713 GZ, Groningen, The Netherlands.; Department of Obstetrics and Gynaecology, Section Reproductive Medicine, University of Groningen, University Medical Centre Groningen, 9713 GZ, Groningen, The Netherlands., Mistry RH; Department of Paediatrics, Centre for Liver, Digestive, and Metabolic Diseases, University of Groningen, University Medical Centre Groningen, 9713 GZ, Groningen, The Netherlands., Anderson JLC; Department of Paediatrics, Centre for Liver, Digestive, and Metabolic Diseases, University of Groningen, University Medical Centre Groningen, 9713 GZ, Groningen, The Netherlands., Hoek A; Department of Obstetrics and Gynaecology, Section Reproductive Medicine, University of Groningen, University Medical Centre Groningen, 9713 GZ, Groningen, The Netherlands., Tietge UJF; Department of Paediatrics, Centre for Liver, Digestive, and Metabolic Diseases, University of Groningen, University Medical Centre Groningen, 9713 GZ, Groningen, The Netherlands. uwe.tietge@ki.se.; Division of Clinical Chemistry, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden. uwe.tietge@ki.se.; Clinical Chemistry, Karolinska University Laboratory, Karolinska University Hospital, SE-141 86, Stockholm, Sweden. uwe.tietge@ki.se.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Sep 06; Vol. 9 (1), pp. 12817. Date of Electronic Publication: 2019 Sep 06.
DOI: 10.1038/s41598-019-49091-3
Abstrakt: High density lipoproteins (HDL) are the main cholesterol carriers in follicular fluid (FF), the natural environment of oocyte development. Additionally, HDL have critical biological functions such as anti-oxidative capacity, which have not been studied in reproduction. Therefore, this study aimed to investigate whether the anti-oxidative function of FF-HDL is associated with fertility outcomes. From 253 women undergoing modified natural cycle (MNC)- IVF at a single academic centre FF and plasma were collected (n = 375 cycles). Anti-oxidative function of FF was mainly attributable to HDL (n = 8; 83%). FF-HDL had a higher anti-oxidative function than plasma HDL (n = 19, P < 0.001) coinciding with increased vitamin E and sphingosine 1 phosphate content (P = 0.028 each). Proteomic analysis indicated no significant differences in major anti-oxidative proteins such as paraoxonase 1, apolipoprotein (apo) A-I or apoA-IV between FF-HDL and matched plasma-HDL (n = 5), while apoC-III, apoE and apoC-II were relatively lower in FF-HDL. Finally, FF-HDL anti-oxidative function was related to a decrease in the odds of the oocyte undergoing normal fertilization, an association that persisted after adjustment for confounders (odds ratio 0.97 (0.93-1), P = 0.041). In conclusion, FF-HDL has considerable anti-oxidative properties that might be relevant for embryo quality.
Databáze: MEDLINE
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