TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells.

Autor:	Wu B; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands., Blot FG; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands., Wong AB; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands., Osório C; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands., Adolfs Y; Department of Translational Neuroscience, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Pasterkamp RJ; Department of Translational Neuroscience, University Medical Center Utrecht, Utrecht University, Utrecht, Netherlands., Hartmann J; Institute of Neuroscience, Technical University Munich, Munich, Germany., Becker EB; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom., Boele HJ; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands., De Zeeuw CI; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands.; Netherlands Institute for Neuroscience, Royal Dutch Academy for Arts and Sciences, Amsterdam, Netherlands., Schonewille M; Department of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2019 Sep 05; Vol. 8. Date of Electronic Publication: 2019 Sep 05.
DOI:	10.7554/eLife.45590
Abstrakt:	Despite the canonical homogeneous character of its organization, the cerebellum plays differential computational roles in distinct sensorimotor behaviors. Previously, we showed that Purkinje cell (PC) activity differs between zebrin-negative (Z-) and zebrin-positive (Z+) modules (Zhou et al., 2014). Here, using gain-of-function and loss-of-function mouse models, we show that transient receptor potential cation channel C3 (TRPC3) controls the simple spike activity of Z-, but not Z+ PCs. In addition, TRPC3 regulates complex spike rate and their interaction with simple spikes, exclusively in Z- PCs. At the behavioral level, TRPC3 loss-of-function mice show impaired eyeblink conditioning, which is related to Z- modules, whereas compensatory eye movement adaptation, linked to Z+ modules, is intact. Together, our results indicate that TRPC3 is a major contributor to the cellular heterogeneity that introduces distinct physiological properties in PCs, conjuring functional heterogeneity in cerebellar sensorimotor integration. Competing Interests: BW, FB, AW, CO, YA, RP, JH, EB, HB, CD, MS No competing interests declared (© 2019, Wu et al.)
Databáze:	MEDLINE
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