[Integron diversity in bla vim-2 -carrying carbapenem-resistant clinical Pseudomonas aeruginosa isolates.]

Autor: Savinova TA; National Medical Research Center for Children's Health Federal state autonomous institution of the Russian Federation Ministry of Health, Russia, 119296, Moscow, Russia., Bocharova YA; National Medical Research Center for Children's Health Federal state autonomous institution of the Russian Federation Ministry of Health, Russia, 119296, Moscow, Russia., Lazareva AV; National Medical Research Center for Children's Health Federal state autonomous institution of the Russian Federation Ministry of Health, Russia, 119296, Moscow, Russia., Chebotar IV; Central Research Institute of Epidemiology of the Federal Service on Customers' Rights Protection and Human Well-being Surveillance, 111123, Moscow, Russia., Mayanskiy NA; Russian Children's Clinical Hospital, Pirogov Russian national Research Medical University of the Russian Federation Ministry of Health, 119571, Moscow, Russia.
Jazyk: ruština
Zdroj: Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2019; Vol. 64 (8), pp. 497-502.
DOI: 10.18821/0869-2084-2019-64-8-497-502
Abstrakt: The growing prevalence of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa in nosocomial pathogen populations has been attributed to their clonal spread, and/or horizontal transfer of MBL determinants in mobile genetic elements, including integrons. To characterize the genetic background of the beta-lactamase VIM-2 encoding gene in the population of carbapenemresistant (Carba-R) P. aeruginosa clinical isolates.The detection of class 1 integrons was performed by PCR. Typing of the class 1 integrons containing the bla VIM gene cassette was performed by the PCR-restriction fragment length polymorphism (RFLP) approach followed by sequencing of variable regions of class 1 integrons. Five types of the bla VIM-2 -carrying integrons were identified: ST654-isolates accounting for more than 50% of the Carba-R population harbored In56; ST235-isolates contained In559 (26% Carba-R isolates); ST111-isolates (19% Carba-R isolates) were characterized by carrying In59-like integron; two ST235-isolates harbored In59 and In249 each. Except In56, carrying the only bla VIM-2 -gene cassette, all other identified integron types harbored the genes of resistance to trimethoprim and/or aminoglycosides. No new types of integrons were identified in the P. aeruginosa clinical isolates. The observed correlation of the integron type with specific STs indicates a clonal dissemination of significant resistance determinant producers - ST111, ST654 and ST235 epidemic lines. The features of the integron variable regions can be used for the epidemiological characterization of clinical P. aeruginosa isolates.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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