Sialic acid on avian erythrocytes.

Autor: Jankowski MD; Los Alamos National Laboratory, Biosecurity and Public Health, Mailstop M888, Los Alamos, NM 87545, United States of America. Electronic address: jankowski.mark@epa.gov., Glaberman SR; University of South Alabama, Department of Biology, Mobile, AL 36688; George Mason University, Department of Environmental Science & Policy, Fairfax, VA 22030. Electronic address: sglaberm@gmu.edu., Kimball DB; Los Alamos National Laboratory, Materials Recovery and Recycling, Mailstop E511, Los Alamos, NM 87545, United States of America. Electronic address: dkimball@lanl.gov., Taylor-McCabe KJ; Los Alamos National Laboratory, National Security and Defense, Mailstop B224, Los Alamos, NM 87545, United States of America. Electronic address: kjmccab@lanl.gov., Fair JM; Los Alamos National Laboratory, Biosecurity and Public Health, Mailstop M888, Los Alamos, NM 87545, United States of America. Electronic address: jmfair@lanl.gov.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology [Comp Biochem Physiol B Biochem Mol Biol] 2019 Dec; Vol. 238, pp. 110336. Date of Electronic Publication: 2019 Aug 30.
DOI: 10.1016/j.cbpb.2019.110336
Abstrakt: Understanding variation in physiological traits across taxa is a central question in evolutionary biology that has wide-ranging implications in biomedicine, disease ecology, and environmental protection. Sialic acid (Sia), and in particular, 5-N-acetylneuraminic acid (Neu5Ac), is chemically bound to galactose and the underlying glycan via α2-3 or α2-6 glycosidic linkage (i.e., Siaα2-3Galactose or Siaα2-6Galactose), conferring two different cell surface structures that affects cell to cell communication and interactions with foreign agents including microparasites and toxins. As an initial step towards understanding variation of Sia across the class Aves, we collected red blood cells (RBCs or erythrocytes) and measured Sia quantity in 76 species and 340 individuals using HPLC-MS/MS and glycosidic linkage type in 24 species and 105 individuals using hemagglutination assay. Although Sia quantity did not, α2-6 glycosidic linkage did exhibit a discernable phylogenetic pattern as evaluated by a phylogenetic signal (λ) value of 0.7. Sia quantity appeared to be higher in after hatch year birds than hatch year birds (P < 0.05); moreover, ~80% of the measured Sia across all individuals or species was expressed by ~20% of the individuals or species. Lastly, as expected, we detected a minimal presence of 5-N-glycolylneuraminic acid in the avian RBCs tested. These data provide novel insights and a large baseline dataset for further study on the variability of Sia in the class Aves which might be useful for understanding Sia dependent processes in birds.
(Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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