Effects of the combination of low-level laser therapy and anionic polymer membranes on bone repair.

Autor: Dos Santos DA; Department of Morphology and Pathology, Faculty of Medicine of Jundiaí, Francisco Telles st., 250, Vila Arens, Jundiaí, São Paulo, 13202-550, Cx Postal 1295, Brazil., de Guzzi Plepis AM; Interunit Postgraduate Program in Bioengineering, University of São Paulo, USP, Trabalhador São Carlense av., 400, São Carlos, São Paulo, Brazil., da Conceição Amaro Martins V; Institute of Chemistry of São Carlos, University of São Paulo, USP, Trabalhador São Carlense av., 400, São Carlos, São Paulo, Brazil., Cardoso GBC; Materials Engineering Department, Faculty of Mechanical Engineering, State University of Campinas, Campinas, São Paulo, Brazil., Santos AR Jr; Center of Natural and Human Sciences, Federal University of ABC, São Bernardo do Campo, São Paulo, Brazil., Iatecola A; Department of Morphology and Pathology, Faculty of Medicine of Jundiaí, Francisco Telles st., 250, Vila Arens, Jundiaí, São Paulo, 13202-550, Cx Postal 1295, Brazil., Andrade TN; Department of Morphology and Pathology, Faculty of Medicine of Jundiaí, Francisco Telles st., 250, Vila Arens, Jundiaí, São Paulo, 13202-550, Cx Postal 1295, Brazil., Monteiro FM; Department of Morphology and Pathology, Faculty of Medicine of Jundiaí, Francisco Telles st., 250, Vila Arens, Jundiaí, São Paulo, 13202-550, Cx Postal 1295, Brazil., Calegari ARA; Department of Morphology and Pathology, Faculty of Medicine of Jundiaí, Francisco Telles st., 250, Vila Arens, Jundiaí, São Paulo, 13202-550, Cx Postal 1295, Brazil., Chacon EL; Department of Morphology and Pathology, Faculty of Medicine of Jundiaí, Francisco Telles st., 250, Vila Arens, Jundiaí, São Paulo, 13202-550, Cx Postal 1295, Brazil., Cunha MR; Department of Morphology and Pathology, Faculty of Medicine of Jundiaí, Francisco Telles st., 250, Vila Arens, Jundiaí, São Paulo, 13202-550, Cx Postal 1295, Brazil. cunhamr@hotmail.com.; Interunit Postgraduate Program in Bioengineering, University of São Paulo, USP, Trabalhador São Carlense av., 400, São Carlos, São Paulo, Brazil. cunhamr@hotmail.com.
Jazyk: angličtina
Zdroj: Lasers in medical science [Lasers Med Sci] 2020 Jun; Vol. 35 (4), pp. 813-821. Date of Electronic Publication: 2019 Aug 29.
DOI: 10.1007/s10103-019-02864-8
Abstrakt: In view of the limitations of bone reconstruction surgeries using autologous grafts as a gold standard, tissue engineering is emerging as an alternative, which permits the fabrication and improvement of scaffolds to stimulate osteogenesis and angiogenesis, processes that are essential for bone repair. Polymers are used to mimic the extracellular bone matrix and support cell growth. In addition, bone neoformation can be induced by external factors such as laser irradiation, which stimulates bone metabolism. The objective of this study was to evaluate the regeneration of bone defects using collagen and elastin membranes derived from intestinal serosa and bovine auricular cartilage combined with low-level laser application. Thirty-six Wistar rats were operated to create a 3-mm defect in the distal metaphysis of the left femur and divided into six groups: G1 (control, no treatment); G2 (laser); G3 (elastin graft), G4 (elastin+laser); G5 (collagen graft); G6 (collagen+laser). The animals were sacrificed 6 weeks after surgery and the femurs were removed for analysis of bone repair. Macroscopic and radiological results showed the absence of an infectious process in the surgical area. This was confirmed by histological analysis, which revealed no inflammatory infiltrate. Histomorphometry showed that the formation of new bone started from the margins of the bone defect and its volume was greater in elastin+laser and collagen+laser. We conclude that newly formed bone in the graft area was higher in the groups that received the biomaterials and laser. The collagen and elastin matrices showed biocompatibility.
Databáze: MEDLINE
Externí odkaz: