Localization of the ultraviolet-sensor Opn5m and its effect on myopia-related gene expression in the late-embryonic chick eye.

Autor:	Kato M; Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan., Sato K; Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan., Habuta M; Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan., Fujita H; Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan., Bando T; Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan., Morizane Y; Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan., Shiraga F; Department of Ophthalmology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan., Miyaishi S; Department of Legal Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan., Ohuchi H; Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan.
Jazyk:	angličtina
Zdroj:	Biochemistry and biophysics reports [Biochem Biophys Rep] 2019 Jul 19; Vol. 19, pp. 100665. Date of Electronic Publication: 2019 Jul 19 (Print Publication: 2019).
DOI:	10.1016/j.bbrep.2019.100665
Abstrakt:	Recent studies show that exposure to ultraviolet (UV) light suppresses ocular elongation, which causes myopia development. However, the specific mechanisms of this process have not been elucidated. A UV-sensor, Opsin 5 (Opn5) mRNA was shown to be present in extraretinal tissues. To test the possibility that UV-signals mediated by Opn5 would have a direct effect on the outer connective tissues of the eye, we first examined the expression patterns of a mammalian type Opn5 (Opn5m) in the late-embryonic chicken eye. Quantitative PCR showed Opn5m mRNA expression in the cornea and sclera. The anti-Opn5m antibody stained a small subset of cells in the corneal stroma and fibrous sclera. We next assessed the effect of UV-A (375 nm) irradiation on the chicken fibroblast cell line DF-1 overexpressing chicken Opn5m . UV-A irradiation for 30 min significantly increased the expression of Early growth response 1 ( Egr1 ), known as an immediate early responsive gene, and of Matrix metalloproteinase 2 ( Mmp2) in the presence of retinal chromophore 11- cis -retinal. In contrast, expression of Transforming growth factor beta 2 and Tissue inhibitor of metalloproteinase 2 was not significantly altered. These results indicate that UV-A absorption by Opn5m can upregulate the expression levels of Egr1 and Mmp2 in non-neuronal, fibroblasts. Taken together with the presence of Opn5m in the cornea and sclera, it is suggested that UV-A signaling mediated by Opn5 in the extraretinal ocular tissues could influence directly the outer connective tissues of the chicken late-embryonic eye.
Databáze:	MEDLINE
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