| Autor: |
Zaccara IM; Universidade Federal do Rio Grande do Sul - UFRGS, School of Dentistry, Graduate program, Porto Alegre, RS, Brazil., Jardine AP; Universidade Federal do Rio Grande do Sul - UFRGS, School of Dentistry, Graduate program, Porto Alegre, RS, Brazil., Mestieri LB; Universidade Federal do Rio Grande do Sul - UFRGS, School of Dentistry, Graduate program, Porto Alegre, RS, Brazil., Quintana RM; Universidade Federal do Rio Grande do Sul - UFRGS, School of Dentistry, Graduate program, Porto Alegre, RS, Brazil., Jesus L; Universidade Federal do Rio Grande do Sul - UFRGS, School of Dentistry, Graduate program, Porto Alegre, RS, Brazil., Moreira MS; Universidade Ibirapuera - UNIB, School of Dentistry, São Paulo, SP, Brazil., Grecca FS; Universidade Federal do Rio Grande do Sul - UFRGS, School of Dentistry, Graduate program, Porto Alegre, RS, Brazil., Martins MD; Universidade Federal do Rio Grande do Sul - UFRGS, School of Dentistry, Graduate program, Porto Alegre, RS, Brazil., Kopper PMP; Universidade Federal do Rio Grande do Sul - UFRGS, School of Dentistry, Graduate program, Porto Alegre, RS, Brazil. |
| Abstrakt: |
This study aimed to evaluate the role of photobiomodulation (PBM) in apexification and apexogenesis of necrotic rat molars with an open apex. Rat molars were exposed to the oral environment for 3 weeks. Canals were rinsed with 2.5% NaOCl and 17% EDTA, filled with antibiotic paste and sealed. After 7 days, canals were rinsed and divided into six groups (n=6): mineral trioxide aggregate (MTA); blood clot (BC); human dental pulp stem cells (hDPSC); MTA+PBM; BC+PBM; and hDPSC+PBM. In hDPSC groups, a 1% agarose gel scaffold was used. Two groups were not exposed: healthy tooth+PBM (n = 6), healthy tooth (n = 3); and one was exposed throughout the experiment: necrotic tooth (n = 3). In PBM groups, irradiation was performed with aluminum gallium indium phosphide (InGaAlP) diode laser for 30 days within 24-h intervals. After that, the specimens were processed for histological and immunohistochemical analyses. Necrotic tooth showed greater neutrophil infiltrate (p < 0.05). Necrotic tooth, healthy tooth, and healthy tooth+PBM groups showed absence of a thin layer of fibrous condensation in the periapical area. All the other groups stimulated the formation of a thicker layer of fibers (p < 0.05). All groups formed more mineralized tissue than necrotic tooth (p < 0.05). PBM associated with MTA, BC, or hDPSC formed more mineralized tissue (p < 0.05). MTA+PBM induced apexification (p < 0.05). Rabbit polyclonal anti-bone sialoprotein (BSP) antibody confirmed the histological findings of mineralized tissue formation, and hDPSC groups exhibited higher percentage of BSP-positive cells. It can be concluded that PBM improved apexification and favored apexogenesis in necrotic rat molars with an open apex. |
