Assembly of Multicomponent Nano-Bioconjugates Composed of Mesoporous Silica Nanoparticles, Proteins, and Gold Nanoparticles.

Autor: Delpiano GR; Department of Chemical and Geological Sciences, University of Cagliari, CSGI, INSTM and CNBS, Cittadella Universitaria, S.S. 554 bivio Sestu, 09042 Monserrato, CA, Italy., Casula MF; Department of Chemical and Geological Sciences, University of Cagliari, CSGI, INSTM and CNBS, Cittadella Universitaria, S.S. 554 bivio Sestu, 09042 Monserrato, CA, Italy., Piludu M; Department of Biomedical Sciences and Department of Physics, University of Cagliari, Cittadella Universitaria, S.S. 554 bivio Sestu, 09042 Monserrato, CA, Italy., Corpino R; Department of Biomedical Sciences and Department of Physics, University of Cagliari, Cittadella Universitaria, S.S. 554 bivio Sestu, 09042 Monserrato, CA, Italy., Ricci PC; Department of Biomedical Sciences and Department of Physics, University of Cagliari, Cittadella Universitaria, S.S. 554 bivio Sestu, 09042 Monserrato, CA, Italy., Vallet-Regí M; Departamento de Quimica Inorganica y Bioinorganica, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigacion Sanitaria Hospital 12 de Octubre i+12, and Centro de Investigacion Biomedica en Red de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Plaza Ramon y Cajal S/N, 28040 Madrid, Spain., Sanjust E; Department of Biomedical Sciences and Department of Physics, University of Cagliari, Cittadella Universitaria, S.S. 554 bivio Sestu, 09042 Monserrato, CA, Italy., Monduzzi M; Department of Chemical and Geological Sciences, University of Cagliari, CSGI, INSTM and CNBS, Cittadella Universitaria, S.S. 554 bivio Sestu, 09042 Monserrato, CA, Italy., Salis A; Department of Chemical and Geological Sciences, University of Cagliari, CSGI, INSTM and CNBS, Cittadella Universitaria, S.S. 554 bivio Sestu, 09042 Monserrato, CA, Italy.
Jazyk: angličtina
Zdroj: ACS omega [ACS Omega] 2019 Jun 25; Vol. 4 (6), pp. 11044-11052. Date of Electronic Publication: 2019 Jun 25 (Print Publication: 2019).
DOI: 10.1021/acsomega.9b01240
Abstrakt: The purpose of this work was the assembly of multicomponent nano-bioconjugates based on mesoporous silica nanoparticles (MSNs), proteins (bovine serum albumin, BSA, or lysozyme, LYZ), and gold nanoparticles (GNPs). These nano-bioconjugates may find applications in nanomedicine as theranostic devices. Indeed, MSNs can act as drug carriers, proteins stabilize MSNs within the bloodstream, or may have therapeutic or targeting functions. Finally, GNPs can either be used as contrast agents for imaging or for photothermal therapy. Here, amino-functionalized MSNs (MSN-NH 2 ) were synthesized and characterized through various techniques (small angle X-rays scattering TEM, N 2 adsorption/desorption isotherms, and thermogravimetric analysis (TGA)). BSA or lysozyme were then grafted on the external surface of MSN-NH 2 to obtain MSN-BSA and MSN-LYZ bioconjugates, respectively. Protein immobilization on MSNs surface was confirmed by Fourier transform infrared spectroscopy, ζ-potential measurements, and TGA, which also allowed the estimation of protein loading. The MSN-protein samples were then dispersed in a GNP solution to obtain MSN-protein-GNPs nano-bioconjugates. Transmission electron microscopy (TEM) analysis showed the occurrence of GNPs on the MSN-protein surface, whereas almost no GNPs occurred in the protein-free control samples. Fluorescence and Raman spectroscopies suggested that proteins-GNP interactions involve tryptophan residues.
Competing Interests: The authors declare no competing financial interest.
Databáze: MEDLINE
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