| Autor: |
Lifsey BJ Jr; Program in Environmental Toxicology, University of Tennessee College of Veterinary Medicine, Knoxville 37901., Farkas WR, Reyniers JP |
| Jazyk: |
angličtina |
| Zdroj: |
Chemico-biological interactions [Chem Biol Interact] 1988; Vol. 68 (3-4), pp. 241-57. |
| DOI: |
10.1016/0009-2797(88)90019-1 |
| Abstrakt: |
Lysinoalanine [N epsilon-(DL-2-amino-2-carboxyethyl)-L-lysine; LAL], a nephrotoxic lysine analog, inhibits the lysyl-tRNA-synthetase (EC 6.1.1.6) of prokaryotic and eukaryotic cells competitively at micromolar concentrations. Incorporation of [14C]lysine into protein by a cell-free eukaryotic protein-synthesizing system was inhibited by LAL. Inhibition was 69.7% and 18.4% at LAL concentrations of 1.0 mM and 0.1 mM, respectively. LAL was incorporated into protein as well as being an inhibitor as indicated by the incorporation of [14C]LAL into protein by the cell-free eukaryote protein-synthesizing system. The proteins labeled with [14C]LAL co-electrophoresed with those labeled with [14C]lysine. These results indicate that LAL is an inhibitor of both prokaryote and eukaryote lysyl-tRNA-synthetase. Furthermore, it is incorporated into protein. Both of these actions can be factors in the nephrotoxicity of this common food contaminant. Possible mechanisms for the toxicity of lysinoalanine are discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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