Molecular insights into the behaviour of bile salts at interfaces: a key to their role in lipid digestion.

Autor: Pabois O; Institut Laue-Langevin, Grenoble 38000, France; Institute of Pharmaceutical Science, King's College London, London SE1 9NH, United Kingdom. Electronic address: olivia.pabois@kcl.ac.uk., Lorenz CD; Department of Physics, King's College London, London WC2R 2LS, United Kingdom. Electronic address: chris.lorenz@kcl.ac.uk., Harvey RD; Institut für Pharmazie, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale) 06099, Germany. Electronic address: richard.harvey@pharmazie.uni-halle.de., Grillo I; Institut Laue-Langevin, Grenoble 38000, France. Electronic address: grillo@ill.fr., Grundy MM; School of Agriculture, Policy and Development, University of Reading, Reading RG6 6AR, United Kingdom. Electronic address: m.m.grundy@reading.ac.uk., Wilde PJ; Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UA, United Kingdom. Electronic address: peter.wilde@quadram.ac.uk., Gerelli Y; Institut Laue-Langevin, Grenoble 38000, France. Electronic address: gerelli@ill.fr., Dreiss CA; Institute of Pharmaceutical Science, King's College London, London SE1 9NH, United Kingdom. Electronic address: cecile.dreiss@kcl.ac.uk.
Jazyk: angličtina
Zdroj: Journal of colloid and interface science [J Colloid Interface Sci] 2019 Nov 15; Vol. 556, pp. 266-277. Date of Electronic Publication: 2019 Aug 05.
DOI: 10.1016/j.jcis.2019.08.010
Abstrakt: Hypotheses: Understanding the mechanisms underlying lipolysis is crucial to address the ongoing obesity crisis and associated cardiometabolic disorders. Bile salts (BS), biosurfactants present in the small intestine, play key roles in lipid digestion and absorption. It is hypothesised that their contrasting functionalities - adsorption at oil/water interfaces and shuttling of lipolysis products away from these interfaces - are linked to their structural diversity. We investigate the interfacial films formed by two BS, sodium taurocholate (NaTC) and sodium taurodeoxycholate (NaTDC), differing by the presence or absence of a hydroxyl group on their steroid skeleton.
Experiments: Their adsorption behaviour at the air/water interface and interaction with a phospholipid monolayer - used to mimic a fat droplet interface - were assessed by surface pressure measurements and ellipsometry, while interfacial morphologies were characterised in the lateral and perpendicular directions by Brewster angle microscopy, X-ray and neutron reflectometry, and molecular dynamics simulations.
Findings: Our results provide a comprehensive molecular-level understanding of the mechanisms governing BS interfacial behaviour. NaTC shows a higher affinity for the air/water and lipid/water interfaces, and may therefore favour enzyme adsorption, whereas NaTDC exhibits a higher propensity for desorption from these interfaces, and may thus more effectively displace hydrolysis products from the interface, through dynamic exchange.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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