Chemical compounds as well as antioxidant and litholytic activities of Arbutus unedo L. leaves against calcium oxalate stones.

Autor: Kachkoul R; Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, University Sidi Mohammed Ben Abdellah, BP 1893, Fez, Morocco; Faculty of Science and Technology, University Sidi Mohammed Ben Abdellah, BP 2202, Fez, Morocco. Electronic address: rabie.kachkoul@usmba.ac.ma., Squalli Housseini T; Laboratory of Molecular Bases in Human Pathology and Therapeutic Tools, Faculty of Medicine and Pharmacy, University Sidi Mohammed Ben Abdellah, BP 1893, Fez, Morocco; Department of Nephrology, University Hospital Hassan II, BP 1835, Atlas, Fez, Morocco., Mohim M; Faculty of Science and Technology, University Sidi Mohammed Ben Abdellah, BP 2202, Fez, Morocco; Laboratory of Molecular Bases in Human Pathology and Therapeutic Tools, Faculty of Medicine and Pharmacy, University Sidi Mohammed Ben Abdellah, BP 1893, Fez, Morocco., El Habbani R; Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, University Sidi Mohammed Ben Abdellah, BP 1893, Fez, Morocco., Miyah Y; Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, University Sidi Mohammed Ben Abdellah, BP 1893, Fez, Morocco., Lahrichi A; Laboratory of Biochemistry, Faculty of Medicine and Pharmacy, University Sidi Mohammed Ben Abdellah, BP 1893, Fez, Morocco.
Jazyk: angličtina
Zdroj: Journal of integrative medicine [J Integr Med] 2019 Nov; Vol. 17 (6), pp. 430-437. Date of Electronic Publication: 2019 Aug 13.
DOI: 10.1016/j.joim.2019.08.001
Abstrakt: Objective: The present study aimed to quantify and identify the bioactive compounds of the Arbutus unedo L. leaves in order to evaluate both their antioxidant properties and litholytic activities against calcium oxalate stones.
Methods: This survey was carried out using hydroalcoholic extract (E.FA) and infusion (I.FA) of A. unedo leaves. The quantification of phenolic compounds, flavonoids, flavonols and anthocyanins was done by spectrophotometric methods and identification of chemical components was performed by ultra-performance liquid chromatography with photodiode array and electrospray ionization tandem mass spectrometry. Antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and by the ferric reducing/antioxidant power (FRAP) assay. Litholytic activity of E.FA and I.FA was studied using a special model that resembles circuitry of the urinary system.
Results: E.FA showed greater antioxidant efficacy than I.FA (P < 0.05). Its higher efficiency was shown via the values of median inhibitory concentration, which was close to (76.14 ± 0.91) µg/mL for E.FA versus (202.64 ± 5.77) μg/mL for I.FA using the DPPH method, and (53.77 ± 0.81) μg/mL for E.FA versus (236.86 ± 31.90) μg/mL for I.FA, using FRAP method. I.FA exhibited significantly higher litholytic activity compared to E.FA (P < 0.05), with dissolution values of 31.03% ± 0.63% versus 14.55% ± 0.65%, respectively.
Conclusion: Overall, the results suggest that the A. unedo is rich in bioactive compounds, and possesses antioxidant and litholitic abilities that are worthy of further study.
(Copyright © 2019 Shanghai Changhai Hospital. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: