| Autor: |
Meerovich GA; Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia. meerovich@nsc.gpi.ru.; National Research Nuclear University 'MEPHI', Moscow, Russia. meerovich@nsc.gpi.ru., Akhlyustina EV; National Research Nuclear University 'MEPHI', Moscow, Russia., Tiganova IG; N.F. Gamaleya National Research Center of Epidemiology and Microbiology, Moscow, Russia., Lukyanets EA; Organic Intermediates and Dyes Institute, Moscow, Russia., Makarova EA; Organic Intermediates and Dyes Institute, Moscow, Russia., Tolordava ER; N.F. Gamaleya National Research Center of Epidemiology and Microbiology, Moscow, Russia., Yuzhakova OA; Organic Intermediates and Dyes Institute, Moscow, Russia., Romanishkin ID; Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia., Philipova NI; N.F. Gamaleya National Research Center of Epidemiology and Microbiology, Moscow, Russia., Zhizhimova YS; N.F. Gamaleya National Research Center of Epidemiology and Microbiology, Moscow, Russia., Romanova YM; N.F. Gamaleya National Research Center of Epidemiology and Microbiology, Moscow, Russia.; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Loschenov VB; Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia.; National Research Nuclear University 'MEPHI', Moscow, Russia., Gintsburg AL; N.F. Gamaleya National Research Center of Epidemiology and Microbiology, Moscow, Russia.; I.M. Sechenov First Moscow State Medical University, Moscow, Russia. |
| Abstrakt: |
Antibacterial photodynamic therapy (APDT) is a promising method of treating local infected foci, in particular, surgical and burn wounds, trophic and diabetic ulcers. Photodynamic inactivation (PDI) is able to effectively destroy bacterial cells without them developing resistance in response to treatment.This work was dedicated to the study of photophysical and antibacterial properties of new photosensitizers (PS) based on polycationic phthalocyanines and synthetic bacteriochlorins for photodynamic inactivation of P. aeruginosa bacteria and their biofilms. Gram-negative bacteria P. aeruginosa are often found in infected wounds, presumably in biofilm state and are characterized by rather low susceptibility to APDT, which is a problem. PS were studied for possible aggregation at various concentrations by means of absorption and fluorescence spectroscopy. The results of studies of the ZnPcChol 8 , (3-PyHp) 4 BCBr 4 and (3-PyEBr) 4 BCBr 4 in water and serum confirm the assumption of a low degree of their aggregation at high concentrations.Consequently, their photodynamic efficiency is high enabling to use these PS at high concentrations to sensitize pathological foci for APDT.It was shown that all the investigated PS had a high efficiency of photodynamic inactivation of Gram-negative bacteria P. aeruginosa, as well as their biofilms. Tetracationic hydrophilic near-infrared photosensitizer (3-PyEBr) 4 BCBr 4 with reduced molecule size had significantly higher efficacy of photodynamic inactivation of P. aeruginosa biofilms compared with other studied photosensitizers. |
