Evaluation of the antileishmanial activity of biodegradable microparticles containing a hexanic eluate subfraction of Maytenus guianensis bark.

Autor: Aragão Macedo SR; Laboratory of Biotechnology Applied to Health, Oswaldo Cruz Foundation, FIOCRUZ Rondônia, Porto Velho, RO, Brazil., Ferreira AS; Laboratory of Biotechnology Applied to Health, Oswaldo Cruz Foundation, FIOCRUZ Rondônia, Porto Velho, RO, Brazil., Biguinati de Barros N; Laboratory of Biotechnology Applied to Health, Oswaldo Cruz Foundation, FIOCRUZ Rondônia, Porto Velho, RO, Brazil., Ulisses de Oliveira Meneguetti D; Laboratory of Research in PhysioPharmacology, Federal University of Acre, UFAC, Rio Branco, AC, Brazil., Facundo VA; Laboratory of Research in PhysioPharmacology, Federal University of Acre, UFAC, Rio Branco, AC, Brazil; Laboratory of Natural Product Chemistry, Federal University of Rondônia, UNIR, Porto Velho, RO, Brazil., Shibayama TY; Ary Pinheiro Base Hospital, Porto Velho, RO, Brazil., Nicolete R; Laboratory of Biotechnology Applied to Health, Oswaldo Cruz Foundation, FIOCRUZ Rondônia, Porto Velho, RO, Brazil; Oswaldo Cruz Foundation, FIOCRUZ Ceará, Eusébio, CE, Brazil. Electronic address: rnicolete@gmail.com.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2019 Oct; Vol. 205, pp. 107738. Date of Electronic Publication: 2019 Aug 20.
DOI: 10.1016/j.exppara.2019.107738
Abstrakt: Leishmaniases, caused by Leishmania spp., are among the most prevalent infectious diseases in the world and their treatment may present high toxicity and side/adverse effects. This study evaluated the antileishmanial activity of the Hexanic Eluate subfraction from Maytenus guianensis bark (HEMg) incorporated in microparticles of PLGA. One batch of microparticles produced contained HEMg (HEMgP) and another contained the PLGA polymer alone (PCTE). The microparticles were characterized in regards to diameter, Zeta potential, encapsulation rate and morphology and their cytotoxicity was evaluated against J774 macrophages. The infection assay employing peritoneal macrophages witth L. amazonensis and cytokine dosages were performed on the cell supernatants. The groups of infected BALB/C mice were treated, euthanized and the parasite load and cytokine production were evaluated. The diameters and zeta potential were: 4 μm and -11.6 mV (PCTE) and 7.8 μm and -26.7 mV (HEMgP). The encapsulation rate was ≅ 15% and the morphology of the particles was spherical and homogeneous. In the infection assay, HEMgP inhibited the amastigotes by 70% (24 h) and 59% (48 h) and induced IL-12 and TNF-α production. HEMg in solution reduced the number of parasites in the lymph nodes by 50% and HEMgP administration increased the levels of IL-12 and TNF-α cytokines in lymph nodes and in the lesion site. When encapsulated, HEMg maintained its antileishmanial activity, but in a more attenuated and sustained form over time, showing promise as complementary/alternative therapy against cutaneous leishmaniasis.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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