| Autor: |
Klepa MS; Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil.; CNPq, SHIS QI 1 Conjunto B, Blocos A, B, C e D, Lago Sul, 71605-001, Brasília, Brazil.; Department of Microbiology, Universidade Estadual de Londrina, C.P. 10011, 86057-970 Londrina, Paraná, Brazil., Urquiaga MCO; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, SBN, Quadra 2, Bloco L, Lote 06, Edifício Capes, 70.040-020, Brasília, Brazil.; Department of Microbiology, Universidade Estadual de Londrina, C.P. 10011, 86057-970 Londrina, Paraná, Brazil.; Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil., Somasegaran P; Formerly with BYOZIME Inc., Saint Joseph, Missouri, USA., Delamuta JRM; CNPq, SHIS QI 1 Conjunto B, Blocos A, B, C e D, Lago Sul, 71605-001, Brasília, Brazil.; Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil., Ribeiro RA; CNPq, SHIS QI 1 Conjunto B, Blocos A, B, C e D, Lago Sul, 71605-001, Brasília, Brazil., Hungria M; CNPq, SHIS QI 1 Conjunto B, Blocos A, B, C e D, Lago Sul, 71605-001, Brasília, Brazil.; Department of Microbiology, Universidade Estadual de Londrina, C.P. 10011, 86057-970 Londrina, Paraná, Brazil.; Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil. |
| Abstrakt: |
Information about the symbionts of legumes of the Caesalpinioideae subfamily is still limited, and we performed a polyphasic approach with three Bradyrhizobium strains-CNPSo 3448 T , CNPSo 3394 and CNPSo 3442-isolated from Chamaecrista fasciculata , a native legume broadly distributed in the USA. In the phylogenetic analysis of both the 16S rRNA gene and the intergenic transcribed spacer, the CNPSo strains were clustered within the Bradyrhizobium japonicum superclade. Multilocus sequence analysis with six housekeeping genes- glnII , gyrB , recA , rpoB , atpD and dnaK -indicated that Bradyrhizobium diazoefficiens is the closest species, with 83 % of nucleotide identity. In the genome analyses of CNPSo 3448 T , average nucleotide identity and digital DNA-DNA hybridization results confirmed higher similarity with B. diazoefficiens , with values estimated of 93.35 and 51.50 %, respectively, both below the threshold of the same species, confirming that the CNPSo strains represent a new lineage. BOX-PCR profiles indicated high intraspecific genetic diversity between the CNPSo strains. In the analyses of the symbiotic genes nodC and nifH the CNPSo strains were clustered with Bradyrhizobium arachidis , Bradyrhizobium forestalis , Bradyrhizobium cajani , Bradyrhizobium kavangense and Bradyrhizobium vignae , indicating a different phylogenetic history compared to the conserved core genes. Other physiological (C utilization, tolerance to antibiotics and abiotic stresses), chemical (fatty acid profile) and symbiotic (nodulation host range) properties were evaluated and are described. The data from our study support the description of the CNPSo strains as the novel species Bradyrhizobiumniftali sp. nov., with CNPSo 3448 T (=USDA 10051 T =U687 T =CL 40 T ) designated as the type strain. |
