Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling.

Autor: Zhao K; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Bleackley M; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Chisanga D; 2Department of Computer Science and Information Technology, La Trobe University, Melbourne, VIC 3086 Australia., Gangoda L; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Fonseka P; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Liem M; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Kalra H; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Al Saffar H; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Keerthikumar S; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia.; 3Cancer Research Division, Peter MacCallum Cancer Centre, Melbourne, VIC 3000 Australia.; 4Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, VIC 3010 Australia., Ang CS; 5Bio21 Institute, University of Melbourne, Melbourne, VIC 3010 Australia., Adda CG; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Jiang L; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Yap K; 6ARC Centre of Excellence in Plant Cell Walls and Adelaide Glycomics, The University of Adelaide, Waite Campus, Urrbrae, SA 5064 Australia., Poon IK; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Lock P; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Bulone V; 6ARC Centre of Excellence in Plant Cell Walls and Adelaide Glycomics, The University of Adelaide, Waite Campus, Urrbrae, SA 5064 Australia., Anderson M; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia., Mathivanan S; 1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086 Australia.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2019 Aug 09; Vol. 2, pp. 305. Date of Electronic Publication: 2019 Aug 09 (Print Publication: 2019).
DOI: 10.1038/s42003-019-0538-8
Abstrakt: Extracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from fks1 ∆ or chs3 ∆ or the vps23 ∆ chs3 ∆ double knockout strain were unable to rescue the yeast cells as compared to vps23 ∆ EVs. Overall, we have identified a potential role for yeast EVs in cell wall remodelling.
Competing Interests: Competing interestsThe authors declare no competing interests.
Databáze: MEDLINE
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