Computational design of three-dimensional RNA structure and function.

Autor: Yesselman JD; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA., Eiler D; Department of Biochemistry and Molecular Genetics, University of Colorado Denver School of Medicine, Aurora, CO, USA., Carlson ED; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA., Gotrik MR; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA., d'Aquino AE; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.; Interdisciplinary Biological Sciences Graduate Program, Northwestern University, Evanston, IL, USA., Ooms AN; Department of Cancer Genetics & Genomics, Stanford University School of Medicine, Stanford, CA, USA., Kladwang W; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA., Carlson PD; Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, USA., Shi X; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA., Costantino DA; Department of Biochemistry and Molecular Genetics, University of Colorado Denver School of Medicine, Aurora, CO, USA., Herschlag D; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA.; Department of Chemistry, Stanford University School of Medicine, Stanford, CA, USA.; Stanford ChEM-H (Chemistry, Engineering, and Medicine for Human Health), Stanford University, Stanford, CA, USA., Lucks JB; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.; Interdisciplinary Biological Sciences Graduate Program, Northwestern University, Evanston, IL, USA., Jewett MC; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.; Interdisciplinary Biological Sciences Graduate Program, Northwestern University, Evanston, IL, USA., Kieft JS; Department of Biochemistry and Molecular Genetics, University of Colorado Denver School of Medicine, Aurora, CO, USA., Das R; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA. rhiju@stanford.edu.; Department of Physics, Stanford University, Stanford, CA, USA. rhiju@stanford.edu.
Jazyk: angličtina
Zdroj: Nature nanotechnology [Nat Nanotechnol] 2019 Sep; Vol. 14 (9), pp. 866-873. Date of Electronic Publication: 2019 Aug 19.
DOI: 10.1038/s41565-019-0517-8
Abstrakt: RNA nanotechnology seeks to create nanoscale machines by repurposing natural RNA modules. The field is slowed by the current need for human intuition during three-dimensional structural design. Here, we demonstrate that three distinct problems in RNA nanotechnology can be reduced to a pathfinding problem and automatically solved through an algorithm called RNAMake. First, RNAMake discovers highly stable single-chain solutions to the classic problem of aligning a tetraloop and its sequence-distal receptor, with experimental validation from chemical mapping, gel electrophoresis, solution X-ray scattering and crystallography with 2.55 Å resolution. Second, RNAMake automatically generates structured tethers that integrate 16S and 23S ribosomal RNAs into single-chain ribosomal RNAs that remain uncleaved by ribonucleases and assemble onto messenger RNA. Third, RNAMake enables the automated stabilization of small-molecule binding RNAs, with designed tertiary contacts that improve the binding affinity of the ATP aptamer and improve the fluorescence and stability of the Spinach RNA in cell extracts and in living Escherichia coli cells.
Databáze: MEDLINE