Fast serotonin voltammetry as a versatile tool for mapping dynamic tissue architecture: I. Responses at carbon fibers describe local tissue physiology.

Autor: Abdalla A; Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina, USA., West A; Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina, USA., Jin Y; Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Saylor RA; Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina, USA., Qiang B; Department of Mathematics and Statistics, Southern Illinois University Edwardsville, Edwardsville, Illinois, USA., Peña E; Department of Statistics, University of South Carolina, Columbia, South Carolina, USA., Linden DJ; Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Nijhout HF; Department of Biology, Duke University, Durham, North Carolina, USA., Reed MC; Department of Mathematics, Duke University, Durham, North Carolina, USA., Best J; Department of Mathematics, The Ohio State University, Columbus, Ohio, USA., Hashemi P; Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina, USA.
Jazyk: angličtina
Zdroj: Journal of neurochemistry [J Neurochem] 2020 Apr; Vol. 153 (1), pp. 33-50. Date of Electronic Publication: 2019 Nov 28.
DOI: 10.1111/jnc.14854
Abstrakt: It is important to monitor serotonin neurochemistry in the context of brain disorders. Specifically, a better understanding of biophysical alterations and associated biochemical functionality within subregions of the brain will enable better of understanding of diseases such as depression. Fast voltammetric tools at carbon fiber microelectrodes provide an opportunity to make direct evoked and ambient serotonin measurements in vivo in mice. In this study, we characterize novel stimulation and measurement circuitries for serotonin analyses in brain regions relevant to psychiatric disease. Evoked and ambient serotonin in these brain areas, the CA2 region of the hippocampus and the medial prefrontal cortex, are compared to ambient and evoked serotonin in the substantia nigra pars reticulata, an area well established previously for serotonin measurements with fast voltammetry. Stimulation of a common axonal location evoked serotonin in all three brain regions. Differences are observed in the serotonin release and reuptake profiles between these three brain areas which we hypothesize to arise from tissue physiology heterogeneity around the carbon fiber microelectrodes. We validate this hypothesis mathematically and via confocal imaging. We thereby show that fast voltammetric methods can provide accurate information about local physiology and highlight implications for chemical mapping. Cover Image for this issue: doi: 10.1111/jnc.14739.
(© 2019 International Society for Neurochemistry.)
Databáze: MEDLINE
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