Embryo presence regulates NODAL/LEFTY2 system in the rat oviduct in vivo.

Autor: Argañaraz ME; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET UNT, and Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, Argentina.; Cátedra de Biología Celular y Molecular, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina., Zampini R; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET UNT, and Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, Argentina.; Cátedra de Biología Celular y Molecular, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina., Apichela SA; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET UNT, and Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, Argentina.; Cátedra de Zootecnia General I, Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina., Barraza DE; Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET UNT, and Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, UNT, San Miguel de Tucumán, Argentina., Angiono G; INITRA (Instituto de Investigación y Tecnología en Reproducción Animal), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, Argentina., Lombardo D; INITRA (Instituto de Investigación y Tecnología en Reproducción Animal), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Molecular reproduction and development [Mol Reprod Dev] 2019 Nov; Vol. 86 (11), pp. 1652-1662. Date of Electronic Publication: 2019 Aug 16.
DOI: 10.1002/mrd.23254
Abstrakt: To gain further insight in the mechanisms of the embryo-maternal dialog in the oviduct, expression of members of the transforming growth factor-β superfamily, NODAL, its inhibitor, LEFTY2, and their coreceptor, CFC1, were studied in the oviduct of 3-day post copula (3 dpc) females with and without embryos (E and NE), pseudopregnant rats (SP3), and in 3-day embryos. Nodal transcripts in SP3 oviducts showed a steady-state relative abundance when compared with proestrus stage and the 3 dpc. In contrast, Lefty2 and Cfc1 relative abundance levels in proestrus and 3 dpc were higher. When comparing E with NE oviducts, Nodal and Lefty2 expression levels decreased, while Cfc1 expression increased in the presence of embryos. Nodal messenger RNA (mRNA) was observed in the embryo, but Lefty2 and Cfc1 transcripts were not found. In addition, an increase in Lefty2 expression coincided with increased levels of matrix metalloproteinases 9 mRNA and protein in the oviduct and in the oviductal fluid, respectively. These observations have shed new light on the relevance of the NODAL/LEFTY2 pathway in the oviduct during early embryo development and the role of the embryo in modulating this pathway.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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