PEG-coated and Gd-loaded fluorescent silica nanoparticles for targeted prostate cancer magnetic resonance imaging and fluorescence imaging.

Autor: Jiang W; Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People's Republic of China., Fang H; Department of Breast Diseases, Chongqing University Cancer Hospital, Chongqing Cancer Institute and Chongqing Cancer Hospital, Chongqing 400030, People's Republic of China., Liu F; Institute of Ultrasound Imaging, Department of Ultrasound, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People's Republic of China., Zhou X; Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People's Republic of China., Zhao H; Department of Gastroenterology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People's Republic of China., He X; Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People's Republic of China., Guo D; Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People's Republic of China.
Jazyk: angličtina
Zdroj: International journal of nanomedicine [Int J Nanomedicine] 2019 Jul 23; Vol. 14, pp. 5611-5622. Date of Electronic Publication: 2019 Jul 23 (Print Publication: 2019).
DOI: 10.2147/IJN.S207098
Abstrakt: Background: Multimodal imaging probes have become a powerful tool for improving detection sensitivity and accuracy, which are important in disease diagnosis and treatment. Methods: In this study, novel bifunctional magnetic resonance imaging (MRI)/fluorescence probes were prepared by loading gadodiamide into fluorescent silica nanoparticles (NPs) (Gd@Cy5.5@SiO 2 -PEG-Ab NPs) for targeting of prostate cancer (PCa). The physicochemical characteristics, biosafety and PCa cell targeting ability of the Gd@Cy5.5@SiO 2 -PEG-Ab NPs were studied in vitro and in vivo. Results: The Gd@Cy5.5@SiO 2 -PEG-Ab NPs had a spherical morphology with a relatively uniform size distribution and demonstrated high efficiency for Gd loading. In vitro and in vivo cell-targeting experiments demonstrated a high potential for the synthesized NPs to target prostate-specific membrane antigen (PSMA) receptor-positive PCa cells, enabling MRI and fluorescence imaging. In vitro cytotoxicity assays and in vivo hematological and pathological assays showed that the prepared NPs exhibited good biological safety. Conclusion: Our study demonstrates that the synthesized Gd@Cy5.5@SiO 2 -PEG-Ab NPs have great potential as MRI/fluorescence contrast agents for specific identification of PSMA receptor-positive PCa cells.
Competing Interests: The authors report no conflicts of interest in this work.
Databáze: MEDLINE
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