Creatine nanoliposome reverts the HPA-induced damage in complex II-III activity of the rats' cerebral cortex.

Autor: Mezzomo NJ; Graduate Program in Nanoscience, Franciscan University, UFN, 1614, Andradas Street, Santa Maria, RS, 97010-032, Brazil. nathanajamillemezzomo@gmail.com., Becker Borin D; Graduate Program in Nanoscience, Franciscan University, UFN, 1614, Andradas Street, Santa Maria, RS, 97010-032, Brazil., Ianiski F; Graduate Program in Nanoscience, Franciscan University, UFN, 1614, Andradas Street, Santa Maria, RS, 97010-032, Brazil., Dotto Fontana B; Graduate Program in Nanoscience, Franciscan University, UFN, 1614, Andradas Street, Santa Maria, RS, 97010-032, Brazil., Diehl de Franceschi I; Graduate Program in Biological Sciences: Toxicological Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, UFRGS, 2600, Ramiro Barcelos Street, Porto Alegre, RS, 90035-003, Brazil., Bolzan J; Graduate Program in Nanoscience, Franciscan University, UFN, 1614, Andradas Street, Santa Maria, RS, 97010-032, Brazil., Garcez R; Graduate Program in Nanoscience, Franciscan University, UFN, 1614, Andradas Street, Santa Maria, RS, 97010-032, Brazil., Grings M; Graduate Program in Biological Sciences: Toxicological Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, UFRGS, 2600, Ramiro Barcelos Street, Porto Alegre, RS, 90035-003, Brazil., Parmeggiani B; Graduate Program in Biological Sciences: Toxicological Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, UFRGS, 2600, Ramiro Barcelos Street, Porto Alegre, RS, 90035-003, Brazil., da Silva Fernandes L; Graduate Program in Nanoscience, Franciscan University, UFN, 1614, Andradas Street, Santa Maria, RS, 97010-032, Brazil., de Almeida Vaucher R; Graduate Program in Nanoscience, Franciscan University, UFN, 1614, Andradas Street, Santa Maria, RS, 97010-032, Brazil., Leipnitz G; Graduate Program in Biological Sciences: Toxicological Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, UFRGS, 2600, Ramiro Barcelos Street, Porto Alegre, RS, 90035-003, Brazil., Duval Wannmacher CM; Graduate Program in Biological Sciences: Toxicological Biochemistry, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, UFRGS, 2600, Ramiro Barcelos Street, Porto Alegre, RS, 90035-003, Brazil., Cielo Rech V; Graduate Program in Nanoscience, Franciscan University, UFN, 1614, Andradas Street, Santa Maria, RS, 97010-032, Brazil. vga.cielo@gmail.com.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2019 Dec; Vol. 46 (6), pp. 5897-5908. Date of Electronic Publication: 2019 Aug 13.
DOI: 10.1007/s11033-019-05023-y
Abstrakt: Phenylketonuria (PKU) is a metabolic disorder accumulating phenylalanine (Phe) and its metabolites in plasma and tissues of the patients. Regardless of the mechanisms, which Phe causes brain impairment, are poorly understood, energy deficit may have linked to the neurotoxicity in PKU. It is widely recognized that creatine is involved in maintaining of cerebral energy homeostasis. Because of this, in a previous work, we incorporated it into liposomes and this increased the concentration of creatine in the cerebral cortex. Here, we examined the effect of creatine nanoliposomes on parameters of oxidative stress, enzymes of phosphoryl transfer network, and activities of the mitochondrial respiratory chain complexes (RCC) in the cerebral cortex of young rats chemically induced hyperphenylalaninemia (HPA). HPA was induced with L-phenylalanine (5.2 µmol/g body weight; twice a day; s.c.), and phenylalanine hydroxylase inhibitor, α-methylphenylalanine (2.4 µmol/g body weight; once a day; i.p.), from the 7th to the 19th day of life. HPA reduced the activities of pyruvate kinase, creatine kinase, and complex II + III of RCC in the cerebral cortex. Creatine nanoliposomes prevented the inhibition of the activities of the complexes II + III, caused by HPA, and changes oxidative profile in the cerebral cortex. Considering the importance of the mitochondrial respiratory chain for brain energy production, our results suggesting that these nanoparticles protect against neurotoxicity caused by HPA, and can be viable candidates for treating patients HPA.
Databáze: MEDLINE
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