The effect of platelet lysate in culture of PDLSCs: an in vitro comparative study.

Autor:	Abuarqoub DA; Cell Therapy Center, The University of Jordan, Amman, Jordan., Aslam N; Cell Therapy Center, The University of Jordan, Amman, Jordan., Barham RB; Cell Therapy Center, The University of Jordan, Amman, Jordan., Ababneh NA; Cell Therapy Center, The University of Jordan, Amman, Jordan., Shahin DA; Cell Therapy Center, The University of Jordan, Amman, Jordan., Al-Oweidi AA; Cell Therapy Center, The University of Jordan, Amman, Jordan., Jafar HD; Cell Therapy Center, The University of Jordan, Amman, Jordan.; School of Medicine, The University of Jordan, Amman, Jordan., Al-Salihi MA; Cell Therapy Center, The University of Jordan, Amman, Jordan., Awidi AS; Cell Therapy Center, The University of Jordan, Amman, Jordan.; School of Medicine, The University of Jordan, Amman, Jordan.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2019 Aug 08; Vol. 7, pp. e7465. Date of Electronic Publication: 2019 Aug 08 (Print Publication: 2019).
DOI:	10.7717/peerj.7465
Abstrakt:	Background: Cellular therapy clinical applications require large-scale production of stem cells. Therefore, abundance, ease of isolation, and proliferative potential are the most important factors in choosing the appropriate source of cells for transplantation studies. Multipotent stem cells obtained from periodontal ligament (PDL) can be used in periodontal tissue regeneration. In this study, we aimed to evaluate and compare the characteristics of periodontal ligament stem cells (PDLSCs), extracted by either enzymatic digestion or explant methods, and expanded using two different serum types: fetal bovine serum (FBS) and xeno-free platelet lysate (PL). Methods: Expanded PDLSCs were assessed for their proliferation capacity, surface markers expression, colony formation, differentiation potential and ability to self-renewal. Most importantly, PDLSCs were evaluated for their ability to produce osteoblasts in vitro . Results: PDLSCs isolated by explant method and expanded in PL serve as a promising source of stem cells for osteoblasts regeneration. These cells showed higher proliferation capacity, they retained their stemness characteristics throughout the passages and they revealed an increase in the expression level of osteogenic markers, without showing any karyotypic abnormalities after cell expansion. Conclusions: PDLSCs produced using explant extraction method and expanded in cell culture media supplemented with PL provide an excellent source of xeno-free cells for the generation of functional osteoblasts. Competing Interests: The authors declare there are no competing interests.
Databáze:	MEDLINE
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