Engineered Interspecies Amino Acid Cross-Feeding Increases Population Evenness in a Synthetic Bacterial Consortium.

Autor:	Ziesack M; Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, Boston, Massachusetts, USA., Gibson T; Massachusetts Host-Microbiome Center, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA., Oliver JKW; Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, Boston, Massachusetts, USA., Shumaker AM; Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, Boston, Massachusetts, USA., Hsu BB; Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA., Riglar DT; Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, Boston, Massachusetts, USA.; Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA., Giessen TW; Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA., DiBenedetto NV; Massachusetts Host-Microbiome Center, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA., Bry L; Massachusetts Host-Microbiome Center, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA., Way JC; Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, Boston, Massachusetts, USA., Silver PA; Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, Boston, Massachusetts, USA Pamela_Silver@hms.harvard.edu ggerber@bwh.harvard.edu.; Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA., Gerber GK; Massachusetts Host-Microbiome Center, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA Pamela_Silver@hms.harvard.edu ggerber@bwh.harvard.edu.
Jazyk:	angličtina
Zdroj:	MSystems [mSystems] 2019 Aug 13; Vol. 4 (4). Date of Electronic Publication: 2019 Aug 13.
DOI:	10.1128/mSystems.00352-19
Abstrakt:	In nature, microbes interact antagonistically, neutrally, or beneficially. To shed light on the effects of positive interactions in microbial consortia, we introduced metabolic dependencies and metabolite overproduction into four bacterial species. While antagonistic interactions govern the wild-type consortium behavior, the genetic modifications alleviated antagonistic interactions and resulted in beneficial interactions. Engineered cross-feeding increased population evenness, a component of ecological diversity, in different environments, including in a more complex gnotobiotic mouse gut environment. Our findings suggest that metabolite cross-feeding could be used as a tool for intentionally shaping microbial consortia in complex environments. IMPORTANCE Microbial communities are ubiquitous in nature. Bacterial consortia live in and on our body and in our environment, and more recently, biotechnology is applying microbial consortia for bioproduction. As part of our body, bacterial consortia influence us in health and disease. Microbial consortium function is determined by its composition, which in turn is driven by the interactions between species. Further understanding of microbial interactions will help us in deciphering how consortia function in complex environments and may enable us to modify microbial consortia for health and environmental benefits. (Copyright © 2019 Ziesack et al.)
Databáze:	MEDLINE
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