Nociceptive-like behavior and analgesia in silver catfish (Rhamdia quelen).

Autor: Rodrigues P; Post-Graduation Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Barbosa LB; Pharmacy School, Federal University of Santa Maria, Santa Maria, RS, Brazil., Bianchini AE; Post-Graduation Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil., Ferrari FT; Pharmacy School, Federal University of Santa Maria, Santa Maria, RS, Brazil., Baldisserotto B; Pharmacy School, Federal University of Santa Maria, Santa Maria, RS, Brazil., Heinzmann BM; Pharmacy School, Federal University of Santa Maria, Santa Maria, RS, Brazil. Electronic address: berta.heinzmann@gmail.com.
Jazyk: angličtina
Zdroj: Physiology & behavior [Physiol Behav] 2019 Oct 15; Vol. 210, pp. 112648. Date of Electronic Publication: 2019 Aug 10.
DOI: 10.1016/j.physbeh.2019.112648
Abstrakt: Fish are useful animal models in research and have been employed in developing new pharmacological approaches. This study aimed to establish the use of silver catfish (Rhamdia quelen) as an animal model to evaluate antinociceptive activity. Initially, different concentrations of acetic acid (2.5-20%), formalin 1% (1-10 μL), menthol 0.5% (1-10 μL) or vehicle were injected in the lips to establish which concentration of each sample promotes nociceptive-like behavior in various parameters. The effect of morphine (0.5-10 mg/kg) on locomotion parameters was also evaluated for antinociceptive concentration determination. Morphine was administered intramuscularly immediately prior to algogen administration. The inhibition was evaluated with the antagonist naloxone (5 mg/kg), which was administered in the same way. Recording time varied according to the algogen used in each test and locomotor activity was evaluated by ANY-maze® software. Acid acetic at 15%, 10 μL of 1% formalin, and 1 μL of 0.5% menthol were chosen since they promoted nociceptive-like behavior in several parameters. Morphine (5 mg/kg) reversed the algogen-induced nociceptive-like behavior and naloxone inhibited this effect. Therefore, the proposed experimental model demonstrated specificity for nociception, since the reversion of the nociceptive-like behavior for a compound with well-described analgesic activity was observed. This new pharmacological model contributes to evaluating compounds with analgesic potential and developing new analgesic drugs, in addition to being a promising alternative to use with rodents.
(Copyright © 2019. Published by Elsevier Inc.)
Databáze: MEDLINE
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