Comparison between in vitro embryo production using Y-sorted sperm and timed artificial insemination with non-sorted sperm to produce crossbred calves.

Autor: de Oliveira Bezerra A; Programa de Pós-Graduação em Ciências Veterinárias, UFMS, Av. Senador Felinto Muller, 2443, 79070-900, Campo Grande, MS, Brazil., Nicacio AC; Embrapa Gado de Corte, Av. Rádio Maia, 830, 79106-550, Vila Popular, Campo Grande, MS, Brazil., de Oliveira Menezes GR; Embrapa Gado de Corte, Av. Rádio Maia, 830, 79106-550, Vila Popular, Campo Grande, MS, Brazil., da Costa Gomes R; Embrapa Gado de Corte, Av. Rádio Maia, 830, 79106-550, Vila Popular, Campo Grande, MS, Brazil., da Silva LOC; Embrapa Gado de Corte, Av. Rádio Maia, 830, 79106-550, Vila Popular, Campo Grande, MS, Brazil., de Souza Rocha-Frigoni NA; UNESP Araçatuba, FMV, Rua Clovis Pestana, 793, Araçatuba, SP, Brazil., Mingoti GZ; UNESP Araçatuba, FMV, Rua Clovis Pestana, 793, Araçatuba, SP, Brazil., da Silva Leão BC; UNESP Araçatuba, FMV, Rua Clovis Pestana, 793, Araçatuba, SP, Brazil., da Costa E Silva EV; Programa de Pós-Graduação em Ciências Veterinárias, UFMS, Av. Senador Felinto Muller, 2443, 79070-900, Campo Grande, MS, Brazil., Nogueira É; Programa de Pós-Graduação em Ciências Veterinárias, UFMS, Av. Senador Felinto Muller, 2443, 79070-900, Campo Grande, MS, Brazil; Embrapa Gado de Corte, Av. Rádio Maia, 830, 79106-550, Vila Popular, Campo Grande, MS, Brazil; Embrapa Pantanal, Rua 21 de Setembro, 1880, 79320-900, Corumbá, MS, Brazil. Electronic address: eriklis.nogueira@embrapa.br.
Jazyk: angličtina
Zdroj: Animal reproduction science [Anim Reprod Sci] 2019 Sep; Vol. 208, pp. 106101. Date of Electronic Publication: 2019 Jun 19.
DOI: 10.1016/j.anireprosci.2019.06.013
Abstrakt: Due to the increasing use of in vitro embryo production (IVEP) and the importance of crossbreeding for beef production, pregnancy rates of Nelore recipients were evaluated following Fixed Time Embryo Transfer with fresh or vitrified IVEP embryos produced with Y-sorted sperm of Angus bulls (B. taurus) or Fixed Time Artificial Insemination using non-sorted sperm. For IVEP in Experiment 1, oocytes were obtained using Ovum Pick Up (OPU) (n = 84 embryos) or from ovaries from a slaughterhouse (SLAUGHTER, n = 66 embryos). In Experiment 2, with oocytes obtained by OPU, IVEP embryos were fresh (FRESH, n = 271) or after vitrification/warming (VITRIFIED, n = 79) and PR was compared with FTAI (n = 239). In Experiment 1, cleavage rates were 63.8% and 39.1% for OPU and SLAUGHTER groups, respectively (P = 0.02), and blastocyst rates were 30.5% and 14.7%, respectively (P = 0.09). The PR was similar when considering the source of oocytes (OPU = 35.7%; SLAUGHTER = 25.8%; P = 0.17). In Experiment 2, there was no difference in PR for FRESH or VITRIFIED embryos (34.3% and 30.4%, respectively, P = 0.72), but lesser than FTAI (47.7, P = 0.002). It is concluded that the IVEP with Y-sorted sperm associated with vitrification or embryos produced with oocytes from different sources did not affect PR when there was transfer of crossbred embryos into recipients, and can optimize large-scale application of IVEP technology; however, FTAI pregnancy rates with non-sex sorted sperm were greater.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: