Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells.

Autor:	Lietzén N; Turku Bioscience Centre, University of Turku and Åbo Akademi University, FI-20520 Turku, Finland., Hirvonen K; Turku Bioscience Centre, University of Turku and Åbo Akademi University, FI-20520 Turku, Finland., Honkimaa A; Faculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland., Buchacher T; Turku Bioscience Centre, University of Turku and Åbo Akademi University, FI-20520 Turku, Finland., Laiho JE; Faculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland., Oikarinen S; Faculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland., Mazur MA; Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm 141 86, Sweden., Flodström-Tullberg M; Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm 141 86, Sweden., Dufour E; Faculty of Medicine and Life Sciences, BioMediTech Institute and Tampere University Hospital, FI-33014 Tampere, Finland., Sioofy-Khojine AB; Faculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland., Hyöty H; Faculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland; Fimlab Laboratories, Pirkanmaa Hospital District, FI-33520 Tampere, Finland., Lahesmaa R; Turku Bioscience Centre, University of Turku and Åbo Akademi University, FI-20520 Turku, Finland. Electronic address: riitta.lahesmaa@utu.fi.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2019 Sep 27; Vol. 19, pp. 340-357. Date of Electronic Publication: 2019 Jul 29.
DOI:	10.1016/j.isci.2019.07.040
Abstrakt:	The group B Coxsackieviruses (CVB), belonging to the Enterovirus genus, can establish persistent infections in human cells. These persistent infections have been linked to chronic diseases including type 1 diabetes. Still, the outcomes of persistent CVB infections in human pancreas are largely unknown. We established persistent CVB infections in a human pancreatic ductal-like cell line PANC-1 using two distinct CVB1 strains and profiled infection-induced changes in cellular protein expression and secretion using mass spectrometry-based proteomics. Persistent infections, showing characteristics of carrier-state persistence, were associated with a broad spectrum of changes, including changes in mitochondrial network morphology and energy metabolism and in the regulated secretory pathway. Interestingly, the expression of antiviral immune response proteins, and also several other proteins, differed clearly between the two persistent infections. Our results provide extensive information about the protein-level changes induced by persistent CVB infection and the potential virus-associated variability in the outcomes of these infections. (Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu