Acute exposure to the water-soluble fraction of gasoline (WSF G ) affects oxygen consumption, nitrogenous-waste and Mg excretion, and activates anaerobic metabolism in the goldfish Carassius auratus.

Autor: G DP; Integrated Group for Aquaculture and Environmental Studies, Dept. of Animal Science, Federal University of Paraná, Curitiba, P.R. 83035-050, Brazil; Programa de Pós-Graduação em Zootecnia, Universidade Federal do Paraná, Curitiba, PR ZIP 80035-050, Brazil; Department of Zoology, University of British Columbia, Vancouver, BC ZIP V6T 1Z4, Canada. Electronic address: giorgi.dalpont@ufpr.br., Souza-Bastos LR; Integrated Group for Aquaculture and Environmental Studies, Dept. of Animal Science, Federal University of Paraná, Curitiba, P.R. 83035-050, Brazil; Programa de Pós-Graduação em Zootecnia, Universidade Federal do Paraná, Curitiba, PR ZIP 80035-050, Brazil; Institute of Technology for Development - Lactec, Curitiba, PR ZIP 81531-980, Brazil. Electronic address: luciana.bastos@lactec.org.br., Giacomin M; Department of Zoology, University of British Columbia, Vancouver, BC ZIP V6T 1Z4, Canada. Electronic address: giacomin@zoology.ubc.ca., Dolatto RG; Grupo de Química Ambiental (GQA), Dept. of Chemistry, Federal University of Paraná, PO Box 19032, Curitiba, PR ZIP 81531-970, Brazil., Baika LM; Grupo de Química Ambiental (GQA), Dept. of Chemistry, Federal University of Paraná, PO Box 19032, Curitiba, PR ZIP 81531-970, Brazil., Grassi MT; Grupo de Química Ambiental (GQA), Dept. of Chemistry, Federal University of Paraná, PO Box 19032, Curitiba, PR ZIP 81531-970, Brazil. Electronic address: mtgrassi@quimica.ufpr.br., Ostrensky A; Integrated Group for Aquaculture and Environmental Studies, Dept. of Animal Science, Federal University of Paraná, Curitiba, P.R. 83035-050, Brazil. Electronic address: ostrensky@ufpr.br., Wood CM; Department of Zoology, University of British Columbia, Vancouver, BC ZIP V6T 1Z4, Canada. Electronic address: woodcm@zoology.ubc.ca.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2019 Dec; Vol. 226, pp. 108590. Date of Electronic Publication: 2019 Aug 09.
DOI: 10.1016/j.cbpc.2019.108590
Abstrakt: Contamination of aquatic environments by petroleum and its products (e.g. gasoline) is a hazard for aquatic organisms as a result of the potential toxicity of monocyclic aromatic hydrocarbons (BTEX) and polycyclic aromatic hydrocarbons (PAH). Our goal was to evaluate the acute effects of the water-soluble fraction of gasoline (WSF G ) on nitrogen excretion, osmoregulation, and metabolism of goldfish Carassius auratus. We first chemically characterized the WSF G and then tested its effects on these physiological aspects of C. auratus, in several different exposure scenarios (0, 0.25, 5, 10 and 25% of WSF G ). The WSF G contained high concentrations BTEX (toluene 70% and benzene 17%) relative to PAH (<1%), and low levels of several metals (Al, Fe, Zn, Sr). Routine O 2 uptake rate (MO 2 ) of goldfish was inhibited by exposure to 5% WSF G , and during post-exposure recovery, MO 2 increased in a dose-dependent fashion. Ammonia excretion was not affected by exposure to WSF G , but urea-N excretion increased progressively with the WSF G concentration. The same pattern of dose/response was observed for net Mg 2+ loss rates and steadily increasing plasma lactate concentrations. Loss rates of Na + , Ca 2+ , K + and Cl - , and plasma concentrations of Mg 2+ and urea-N were not significantly altered. We propose that acute exposure to WSF G inhibits aerobic metabolism and activates anaerobic metabolism, breaking down ATP such that bound Mg 2+ is liberated and the purine ring component is metabolized to urea-N, both of which are subsequently excreted.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: